<?xml version='1.0' encoding='UTF-8'?><?xml-stylesheet href="http://www.blogger.com/styles/atom.css" type="text/css"?><feed xmlns='http://www.w3.org/2005/Atom' xmlns:openSearch='http://a9.com/-/spec/opensearchrss/1.0/' xmlns:georss='http://www.georss.org/georss' xmlns:gd='http://schemas.google.com/g/2005' xmlns:thr='http://purl.org/syndication/thread/1.0'><id>tag:blogger.com,1999:blog-3911178684277112424</id><updated>2011-04-21T14:31:16.487-07:00</updated><title type='text'>Sex Home</title><subtitle type='html'></subtitle><link rel='http://schemas.google.com/g/2005#feed' type='application/atom+xml' href='http://tj080816.blogspot.com/feeds/posts/default'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default?max-results=100'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/'/><link rel='hub' href='http://pubsubhubbub.appspot.com/'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author><generator version='7.00' uri='http://www.blogger.com'>Blogger</generator><openSearch:totalResults>32</openSearch:totalResults><openSearch:startIndex>1</openSearch:startIndex><openSearch:itemsPerPage>100</openSearch:itemsPerPage><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-6692869286975797479</id><published>2009-03-17T22:00:00.000-07:00</published><updated>2009-04-16T03:31:09.107-07:00</updated><title type='text'>淘宝客</title><content type='html'>&lt;a href="http://s.click.alimama.com/a/qXMWq7JdMqA=-10105824"&gt;扫皇打妃---“有一套”超酷手机套专卖&lt;/a&gt;&lt;br /&gt;&lt;br /&gt;&lt;a href="http://s.click.alimama.com/a/qXMXd9fFHWs=-10105824"&gt;蜜与糖---【北京商盟】☆蜜语堂☆立体拼装世界&lt;/a&gt;&lt;br 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src='https://blogger.googleusercontent.com/tracker/3911178684277112424-6692869286975797479?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/6692869286975797479'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/6692869286975797479'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2009/03/blog-post.html' title='淘宝客'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-5756136371397452302</id><published>2008-11-07T07:43:00.001-08:00</published><updated>2009-04-10T08:58:03.764-07:00</updated><title type='text'>a signal of liver cancer</title><content type='html'>abdominal pain, to fall, hematochezia all people over the age of 30 appear abdominal discomfort, abdominal pain, bloating, changes in bowel habits, and a sense of Taipiandaixue fall, and then appeared anemia, fatigue, abdominal mass touch, consideration should be given The possibility of colorectal cancer. One site was along the intestinal limitations of intermittent abdominal pain colorectal cancer is the first warning signal. Taibiandaixue with a sense of falling significantly, the cancer is often a signal.  &lt;br /&gt;        You Lei, under pain under Youlei pain, often referred to the Department of right upper quadrant pain in the liver area, the site of pain is commonly found in hepatitis, cholecystitis, liver cirrhosis, liver cancer, and so on. Oc2008年10月9日cult liver cancer onset, rapidly, and some patients Youlei Tong Tong under sustained a few months after being diagnosed with liver cancer. Therefore, under pain should Youlei as a signal of liver cancer.&lt;br /&gt;irritating cough and Cough or Bu Yu Xue Tan of lung cancer growth in the number of bronchial wall, due to the growth of cancer cells, the destruction of normal tissue structure, a strong stimulus bronchial, causing coughing. After antibiotics, cough medicine can not be eased, and gradually increase the occasional occurrence of chest pain and Xue Tan. Such irritating cough is often considered to be early signs of lung cancer.        breast tumor normal female breasts, soft mass. If you touch to the mass, age and women over the age of 40 should consider the possibility of breast cancer.        abnormal vaginal bleeding and normal menstrual women once a month, usually no bleeding. If the bleeding after sexual intercourse, may be suffering from Cervical signal. Bleeding after sexual intercourse in general has very few, if attention could find cancer early.         blood nose bloody nose mainly in the nose with a small amount of blood, especially in early morning blood nose, nasopharyngeal cancer is often an important signal, in addition to nasopharyngeal blood nose, but also often nose Cyprus, which is due to the oppression caused by pieces of nasopharyngeal carcinoma patients. If the Eustachian tube cancer oppression, will appear tinnitus, so bloody nose, nasal obstruction, tinnitus and headaches in particular the side of migraine, is the risk of nasopharyngeal cancer occurred signal.  &lt;br /&gt;        abdominal pain, to fall, hematochezia all people over the age of 30 appear abdominal discomfort, abdominal pain, bloating, changes in bowel habits, and a sense of Taipiandaixue fall, and then appeared anemia, fatigue, abdominal mass touch, consideration should be given The possibility of colorectal cancer. One site was along the intestinal limitations of intermittent abdominal pain colorectal cancer is the first warning signal. Taibiandaixue with a sense of falling significantly, the cancer is often a signal. &lt;br /&gt;        You Lei, under pain under Youlei pain, often referred to the Department of right upper quadrant pain in the liver area, the site of pain is commonly found in hepatitis, cholecystitis, liver cirrhosis, liver cancer, and so on. Oc2008年10月9日cult liver cancer onset, rapidly, and some patients Youlei Tong Tong under sustained a few months after being diagnosed with liver cancer. Therefore, under pain should Youlei as a signal of liver cancer.&lt;br /&gt;&lt;br /&gt;&lt;iframe frameborder="0" marginheight="0" marginwidth="0" border="0" id="alimamaifrm" name="alimamaifrm" scrolling="no" height="3347px" width="100%"src="http://gouwu.alimama.com/channel/channelCode.htm?pid=mm_11887358_0_0"&gt;&lt;/iframe&gt;&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-5756136371397452302?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/5756136371397452302'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/5756136371397452302'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/11/signal-of-liver-cancer.html' title='a signal of liver cancer'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-7049239875479576703</id><published>2008-10-31T23:29:00.000-07:00</published><updated>2008-11-01T00:39:33.313-07:00</updated><title type='text'>甄志丹周迅赵薇陈坤孙俪版高清晰《画皮》下载地址</title><content type='html'>&lt;span style="font-size:180%;"&gt;新版高清晰《画皮》甄志丹周迅赵薇陈坤主演下载地址&lt;br /&gt;&lt;br /&gt;甄志丹周迅赵薇陈坤版高清晰《画皮》下载地址：有效期10天&lt;br /&gt;&lt;br /&gt;提取码 8f89fd75&lt;br /&gt;&lt;/span&gt;&lt;br /&gt;&lt;a href="http://mail.qq.com/cgi-bin/ftnExs_download?k=5c663839e94cb6e0f2a6813d4064051a5c550d58515c550c15035d5d044903045a57155b030502185e07010c03500e570c5f080d665437e5fac78e82cba293948f3d8bfba6c01be3e4b78015b3b0e98414b3dfeeb5d19319f389e18a3bb2ed1b1648164b0b125535&amp;amp;t=exs_ftn_download&amp;amp;code=8f89fd75"&gt;http://mail.qq.com/cgi-bin/ftnExs_download?k=5c663839e94cb6e0f2a6813d4064051a5c550d58515c550c15035d5d044903045a57155b030502185e07010c03500e570c5f080d665437e5fac78e82cba293948f3d8bfba6c01be3e4b78015b3b0e98414b3dfeeb5d19319f389e18a3bb2ed1b1648164b0b125535&amp;amp;t=exs_ftn_download&amp;amp;code=8f89fd75&lt;/a&gt;&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-7049239875479576703?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/7049239875479576703'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/7049239875479576703'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/blog-post.html' title='甄志丹周迅赵薇陈坤孙俪版高清晰《画皮》下载地址'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-530257381510775885</id><published>2008-10-27T04:46:00.003-07:00</published><updated>2008-10-27T04:46:49.768-07:00</updated><title type='text'>dallas criminal attorney</title><content type='html'>Examples of Criminal Case / Government Investigation Results&lt;br /&gt;1. DISMISSAL – Northern District of Texas, Dallas Division, U.S. v. W.A. Extortion case – conspiracy to extort money and transmitting interstate communications with intent to extort money. Case was dismissed after Indictment for these extortion charges. The defendant/client was indicted based upon allegations that he stole sensitive material regarding investors from a company and then offered to sell the information for one million dollars. The United States Attorney’s Office dismissed the case after defense presentations which showed no criminal intent by the defendant.&lt;br /&gt;2. NO INDICTMENT / INVESTIGATION CLOSED – Northern District of Texas, Dallas Division, re T.H. Bank fraud and false statements to a financial institution. Investigation concerned real estate closings with allegations of inflated appraisals and inflated income/cash flow on loan applications and closing statements. After various meetings with the United States Attorney’s Office and agents of the Federal Bureau of Investigation (FBI), and presentations of evidence to the U.S. Attorney’s Office, the investigation as to this target/defendant was closed.&lt;br /&gt;3. NO INDICTMENT / INVESTIGATION CLOSED – Northern District of Texas, Dallas Division, re H.K. Fraud, Bribery and False Statements to an Agency. Investigation concerned allegations that the target/client, an employee of the Social Security Administration, was obtaining Social Security benefits/monies for persons who would pay him to do so. After various contacts with and presentations to the United States Attorney and the agent for the Office of the Inspector General, the U.S. Attorney’s Office declined prosecution.&lt;br /&gt;4. NO INDICTMENT – Western District of Texas, re M.H. Bribery of Officials. Investigation of monies to school board members in exchange for contracts for services to the local school district. After discussions and presentations to the United States Attorney, the client is not indicted, but will be a witness if necessary.&lt;br /&gt;5. NO INDICTMENT / INVESTIGATION CLOSED – Northern District of Texas, re D.B. Transmission of Pornographic Images/Possession of Underage Images. After discussions and presentations to the United States Attorney’s Office, the investigation was closed with no action.&lt;br /&gt;6. ACQUITTAL (NOT GUILTY VERDICT) – Eastern District of Texas. Client was charged in a conspiracy to distribute cocaine, methamphetamine and marijuana. Seven defendants pled guilty and were sentenced to lengthy prison sentences, and three went to trial. The jury convicted two defendants, but acquitted the client represented by John Teakell and David Finn on all counts.&lt;br /&gt;7. DISMISSAL – Northern District of Texas. Client was indicted for attempting to transport a firearm on a commercial airline flight. The handgun, in client’s checked luggage, accidentally discharged and shot a baggage handler at Dallas/Fort Worth International Airport. After defense counsel reviewed the evidence with the U.S. Attorney’s Office and made a defense presentation, the U.S. Attorney’s Office dismissed the Indictment.&lt;br /&gt;8. DISMISSAL / DIVERSION – Northern District of Texas. Client was indicted for allegedly defrauding the United States Department of Agriculture, specifically, a food and child care program. The U.S. Attorney’s Office dismissed the case before trial after a compelling defense presentation, as the federal prosecutors were not aware of certain evidence that was to be used at trial by the client. The client was on a pre-trial diversion program.&lt;br /&gt;9. IMMUNITY / NO INDICTMENT – Northern District of Texas. Client was a target of a federal investigation for bank fraud and false statements to a financial institution regarding a company’s loan through the U.S. Department of Housing and Urban Development (HUD). After presentations to the U.S. Attorney’s Office, the client was not indicted, but was given immunity from prosecution. The other target was indicted and convicted.&lt;br /&gt;10. NO INDICTMENT / INVESTIGATION CLOSED – Eastern District of Texas. Client was the target of a federal investigation for illegal sales of military equipment. After a defense counsel presentation to the U.S. Attorney’s Office, the prosecution closed the investigation with no indictment.&lt;br /&gt;11. NO INDICTMENT / INVESTIGATION CLOSED – Northern District of Texas. Client was a potential target of a mail fraud scheme in North Texas and elsewhere. After discussions with the presentations to federal agents, the agency declined to refer this client to the U.S. Attorney’s Office for prosecution.&lt;br /&gt;12. NO INDICTMENT / INVESTIGATION CLOSED – Northern District of Texas. Client, a manager of an apartment complex, was a target of a federal investigation for drug trafficking and maintaining a house where drugs are kept or sold. After defense presentation and discussions with the U.S. Attorney’s Office, the prosecution declined to proceed against the client.&lt;br /&gt;13. NO INDICTMENT / INVESTIGATION CLOSED – Eastern District of Texas. Client, a travel agency owner and operator, was the target of a federal investigation for bank fraud and mail fraud. The U.S. Attorney’s Office investigated allegations of fraud for cancelled trips and lost monies after “9/11” restricted booking availability abroad, and declined to prosecute after defense presentations the U.S. Attorney’s Office.&lt;br /&gt;14. NO INDICTMENT / NO TARGET – Eastern District of Texas. Client was considered a subject of an investigation for mail fraud and securities fraud, but was later removed from consideration for prosecution after discussions and presentations to the U.S. Attorney’s Office and investigating authorities.&lt;br /&gt;15. REDUCED CHARGE – Western District of Texas. Client was indicted for possessing and passing counterfeit currency after a U.S. Secret Service investigation with the U.S. Attorney’s Office. After preparing for trial and making defense presentations to the U.S. Attorney’s Office, the charge was reduced to a misprision of a felony -- failure to apprise law enforcement of an on-going felony. The client pled guilty and received probation. A conviction on the counterfeit currency charges would have normally resulted in federal prison time.&lt;br /&gt;16. DEFENDANT RESPONSIBLE FOR LESS THAN GOVERNMENT CLAIMED – Eastern District of Texas. Client proceeded to trial on this drug trafficking Indictment and was convicted, although the client was responsible for much less cocaine than the government alleged. The government’s witnesses originally alleged that the client was involved in at least one hundred (100) kilograms of cocaine, but the jury found that the client was responsible for only 500 grams to less than five (5) kilograms of cocaine.&lt;br /&gt;17. SECURITY CLEARANCE NOT REVOKED – U.S. Department of Defense, Office of Hearings and Appeals. Administrative action sought revocation of a security clearance issued by the Department of the Navy for an employee of a company engaging in providing and maintaining weapons systems equipment and communications. After a trial, the administrative judge declined to revoke the security clearance.&lt;br /&gt;18. NO INDICTMENT / NO TARGET – District of Columbia. In re GG. Client was targeted in a communications company fraud investigation for mail fraud and wire fraud, concerning unsolicited emails forwarded to persons and the content thereof. This criminal investigation began following an investigation by the Securities and Exchange Commission. After the defense demonstrated that Client was not responsible for the email transmissions, but rather only sold the lines and communication capabilities, the U.S. Attorney’s Office removed him as a target in the investigation.&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-530257381510775885?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/530257381510775885'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/530257381510775885'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/dallas-criminal-attorney.html' title='dallas criminal attorney'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-2736679762559228374</id><published>2008-10-27T04:46:00.001-07:00</published><updated>2008-10-27T04:46:24.848-07:00</updated><title type='text'>accident attorney michigan</title><content type='html'>My letter to the Michigan Attorney General&lt;br /&gt;This is the letter I sent off to the Attorney General today. I just wanted to warn as many people as I can that this is happening. I believe this must be some group from overseas, because of the way the number shows up on my caller id. While I was speaking to this person, I could hear lots of other people talking in the background, it sounds like a typical telemarketer boiler room.&lt;br /&gt;I am writing to you because I have received phone calls from these people twice this past week, and I am concerned that someone will fall for this attempt at identity theft.&lt;br /&gt;The first time they called, I refused to answer any questions with sensitive, personal information, hoping they would just stop. Instead, I received another call from them this evening (a different operator), so instead of refusing I simply made up answers to see just what sorts of questions they would ask. It was amazing how blatant they were.&lt;br /&gt;The number shows up on my caller id as 999-999-1234. The caller has a thick accent, and the connection is very 'crackly', like a cell phone in a bad area. They already have my name and address when they call.&lt;br /&gt;They told me they were calling from "Washington Financial Grant Services", and that they have been contacted by the "Federal &amp;amp; State Grant Commission" to distribute grants. I have already been approved for an $8000 grant, simply because I have been paying taxes 'all these years'. The schpiel is that the government puts 10% of all of our taxes into this fund, and the government picks random taxpayers to give 'grants' to out of this fund. Of course, it's a "once in a lifetime" thing and the deadline is "today" (even though they have now called me twice). They even gave me another 'customer service' number that I could call if I had any questions after the call, it was 866-623-4123.&lt;br /&gt;They want to send me 'documents' to claim my grant, but before they can they need to 'verify' that my information is accurate and that I really do have an account. They keep insisting that they'd hate to see me 'lose my money', and keep telling me it happens all the time.&lt;br /&gt;They asked my Date of Birth, How long have I lived at this address, How long have I had this phone number, My bank's name and street address, the bank tracking number, my account number and my mother's maiden name.&lt;br /&gt;I'm afraid it started to fall apart by this time, since I was making up answers (there was no way I'd give them anything even close to the true answers), but apparently they have a way to verify the bank's tracking number and they (of course) couldn't verify the phony number I gave them. When I offered to simply take the papers to the bank and let the bank fill in the correct numbers, the caller first got upset, then the connection finally went dead.&lt;br /&gt;If you have any further questions that I may be able to help with, you may call me, my cell number is xxx-xxx-xxxx.&lt;br /&gt;Hopefully, you will issue an alert regarding this, I am concerned that someone will fall for this obvious scam.&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-2736679762559228374?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/2736679762559228374'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/2736679762559228374'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/accident-attorney-michigan.html' title='accident attorney michigan'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-3835939633622019253</id><published>2008-10-27T04:45:00.003-07:00</published><updated>2008-10-27T04:45:57.641-07:00</updated><title type='text'>life assurance online</title><content type='html'>What is Life Insurance?&lt;br /&gt;Life Insurance (also known as Life Assurance, Term Insurance, term assurance) is a simple way to protect your family financially in the event of your death. If you die during the term of the policy or suffer from a terminal illness1 then the policy will pay out a lump sum of money to your family or nominated beneficiaries.&lt;br /&gt;1A terminal illness is usually classed as having less than 12 months to live. This may differ between providers. Most providers don't cover terminal illness in the last 18 months of a plan.&lt;br /&gt;What is the age restriction on Life Insurance?&lt;br /&gt;After years of waiting, it finally came into effect in the early part of June 2007.&lt;br /&gt;The FSA rule restricting the sale of term assurance past the age of age 70 has been scrapped. This is great news for both Top Quote UK and it's customers across the UK.&lt;br /&gt;For further reading on Over 50's, Over 60's and Over 70's Life Insurance, please read the following articles from Top Quote Online.&lt;br /&gt;Over 50 Life Insurance and Over 60 Life Insurance&lt;br /&gt;Over 70 Life Insurance - Over 70s Rule Scrapped by FSA&lt;br /&gt;How Much Life Insurance do I Need?&lt;br /&gt;You can decide how much Life Cover you require, or you can contact one of our experienced advisors on 0808 178 2777. Many people take out Life Insurance to cover debts and liabilities, for example mortgages. It is also common to take out insurance to cover your family's finances, for example four times your annual salary would mean that your family could continue their standard of living for four years.&lt;br /&gt;For example: A married man with two dependant children, a repayment mortgage of ￡100,000 and an annual salary ￡24,000 may consider taking out the following:&lt;br /&gt;A Mortgage Life Insurance policy for ￡100,000 (which will decrease over the term of the mortgage).&lt;br /&gt;A Level Term Life Insurance policy for ￡96,000 (equal to 4 x Salary, this benefit will not decrease).&lt;br /&gt;How Much Will Life Insurance Cost Me?&lt;br /&gt;Life Insurance premiums are calculated based on your age, gender, smoker status, medical history, height/weight and the amount/type of cover that you choose.&lt;br /&gt;Please take alook at the Top Quote Online Article on Cheap Life Insurance and Affordable Life Insurance by clicking the link below Cheap Life Insurance  Affordable Life Insurance&lt;br /&gt;You can request a quote from our website now by clicking here. This will give you a quote based on your age, gender, smoker status and the amount/type of cover that you choose. Medical history and height/weight will be taken into considering upon receipt of an application, please feel free to contact us for further information.&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-3835939633622019253?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/3835939633622019253'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/3835939633622019253'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/life-assurance-online.html' title='life assurance online'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-5682521395669285998</id><published>2008-10-27T04:45:00.001-07:00</published><updated>2008-10-27T04:45:16.055-07:00</updated><title type='text'>equity credit line</title><content type='html'>Getting to know through this equity credit line article more information on this subject will help you more in time than you may realize, till the opportunity shows up when you really require it.&lt;br /&gt;A home equity loan is effectively a sort of second mortgage. You`ll be borrowing money against the equity you have accumulated on your residential property.&lt;br /&gt;The best-known class of home loan is a `closed-end` home equity loan. This category of home loan makes if possible for you to be loaned a certain amount against the value of your home. You cannot borrow more money on the same equity loan, so if you need more funds subsequently, your only option will be to acquire one more equity loan.&lt;br /&gt;Many people realize that acquiring a home loan on line could help free them from debt. Since you`re borrowing money against your residential property, you`ll find yourself paying a lower rate of interest than you are accustomed to. This`ll almost certainly result in a significantly smaller monthly payment compared to what you would pay for most other loans, yet it is a great deal more risky.&lt;br /&gt;In case you`re in considerable debt and have multiple high interest payments to make each month, you could get cash resources by means of an equity loan in order to finish repaying your other financial dues. You`ll be in a position to efficiently fuse all of your financial commitments into a single low-interest monthly installment.&lt;br /&gt;It`s crucial that you make doubly sure that you can come up with the monthly payments when you procure a home loans. All said and done, if you begin to skip payments, you might lose your residential property. For this reason, you should carry out an extremely meticulous review of your monetary circumstances before you apply for the house loans.&lt;br /&gt;The other equity home loan is a home equity line of credit or HELOC. The most significant distinction, though, is that a home equity line of credit will let you take a loan of more funds with your home as collateral if called for - in some cases, upto a hundred and twenty-five percent of your residential property`s equity.&lt;br /&gt;A mortgage loan is better in most cases; nevertheless, the HELOC is a sound plan if you`re uncertain about just how much money you need to take on loan right away. With the HELOC, you will be able to increase the sum of money you have borrowed against your house.&lt;br /&gt;A house equity loan could be right for you when you feel you should fuse financial debts without delay, and if you`re certain you will be able to fully repay the mortgage online without defaulting on any one of your monthly installments. In case you`re procuring the loan to merge all your debts, ensure that you have the self-control to avail of the total loan toward that essential end.&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-5682521395669285998?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/5682521395669285998'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/5682521395669285998'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/equity-credit-line.html' title='equity credit line'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-600261199057634211</id><published>2008-10-27T04:44:00.001-07:00</published><updated>2008-10-27T04:44:26.067-07:00</updated><title type='text'>fx online trading</title><content type='html'>The internet has become a valuable tool when it comes to trading and investing, and online FX trading is both convenient and accessible. The benefit of using the internet for online trading is that it allows you to view how the market is functioning in real time and to make your Forex trading successful, you need to make sure you have these real time capabilities to help you make quick decisions.&lt;br /&gt;There is an art to online fx trading and there are plenty of companies that help make the process easier; their goal is to give you the resources necessary to take full advantage of this kind of trading.&lt;br /&gt;Online FX Trading Basics&lt;br /&gt;To do online Forex trading, you need both a reliable internet connection and knowledge of the Foreign Exchange Market. Without knowledge of this market, you will be ill equipped to be successful at FX trading.&lt;br /&gt;The foreign exchange market deals with buying and selling currencies throughout the world, and the market is on a 24 hour clock - this fact alone makes online FX trading a valuable option. Your online efforts can be continually monitored making this global trading more successful.&lt;br /&gt;If you are considering getting started with Forex trading, keep in mind that as with any form of investing or online trading, adequate research is a key point, because you want to make sure you understand what you need to do to be successful. You may want to hire a firm that specialize in the foreign exchange market and will guide you through the process of online trading.&lt;br /&gt;Trading Currencies&lt;br /&gt;Most of the trading that is done with FX trading occurs within the realm of a few common currencies which see the majority of the transactions and are called the majors. The currencies include the US Dollar, Japanese Yen, British Pound, Euro, Canadian Dollar, and the Australian Dollar. There is the potential to make a considerable amount of money but you need to watch carefully for market and price fluctuations - this is why trading Forex can be invaluable.&lt;br /&gt;Using Software&lt;br /&gt;If you dont wish to hire a firm to assist you with online FX trading, there are plenty of software programs out there that you can use to help. These software programs are invaluable and a good one will include multiple features that will help make your online trading efforts a success.&lt;br /&gt;A good software program will provide you with instant access to the Global Foreign Exchange market, and will also offer automated alerts as to the market condition and whether or not you should buy or sell in a particular trade. A good idea is to make a list of the software programs available and then research which are the best for your situation.&lt;br /&gt;This article was posted on September 13, 2006&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-600261199057634211?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/600261199057634211'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/600261199057634211'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/fx-online-trading.html' title='fx online trading'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-2670656402039551357</id><published>2008-10-27T04:43:00.001-07:00</published><updated>2008-10-27T04:43:40.727-07:00</updated><title type='text'>equity home line</title><content type='html'>Trump casinos: The art of the comeback&lt;br /&gt;ATLANTIC CITY, N.J. - For months, a tusk on one of the elephants outside the Trump Taj Mahal Casino Resort was missing, probably snapped off by a vandal.&lt;br /&gt;It was one small repair on a long list of things Trump Entertainment Resorts Inc. had to do to spruce up its three casinos in the battle against a growing list of better-financed, newer, flashier casinos changing the face of Atlantic City.&lt;br /&gt;Now the tusk is glued back on — a symbol that the company realizes that the small things are just as important as the big ones when it comes to succeeding in the new Atlantic City.&lt;br /&gt;"You have a lot of competition to Atlantic City now, which it's never had before," said company chairman Donald Trump. "Now it's really a question of marketing and running (the Trump casinos) beautifully."&lt;br /&gt;The company faces stiff headwinds — more than $1.5 billion in debt, better-financed competitors with a big head start on offering the kind of Las Vegas-style resort appeal that Atlantic City has only recently embraced, and at least two deep-pocketed companies who will open new casinos within four years.&lt;br /&gt;It sought buyers for the Taj Mahal, Trump Plaza Hotel and Casino, and Trump Marina Hotel Casino. A proposed deal came close but fell through in July.&lt;br /&gt;Donald Trump said there are still several potential buyers, either for the company as a whole or for a piecemeal sale. But he refuses to identify them or characterize how serious the talks have become.&lt;br /&gt;In the meantime, the company is focused on turning around its bottom line.&lt;br /&gt;"Our turnaround strategy has always been to identify our better customers and market to them," said Mark Juliano, a 30-year veteran casino executive who once ran Caesars Palace in Las Vegas and who took over as Trump's chief executive this summer. "It's to try to get the company away from depending on the day-trip gambler as opposed to someone who wants to come for a two- or three-day entertainment experience."&lt;br /&gt;To do that, Trump needs more hotel rooms to put them in. The company is building a second tower at the Taj Mahal for $255 million that should be open in the third quarter of 2008. The 786 rooms it includes will give Trump 3,670 rooms — about the same as a new casino planned here by Revel Entertainment Group LLC.&lt;br /&gt;The new rooms at the Taj are a major part of the company's strategy to whittle away the $1.5 billion in debt that has limited its options in the past and scared off some potential buyers. The debt remains despite a 2005 bankruptcy reorganization.&lt;br /&gt;Virtually every room in all three casinos has been renovated in recent years, and new restaurants opened at the Taj and Marina. A makeover stripped much of the garishness from the Taj, including its infamous purple carpet, which Juliano promised the state Casino Control Commission "has been banished forever."&lt;br /&gt;Brian Fennell of Lumberton, N.J., stayed at the Taj recently for a trade show, and liked what he saw.&lt;br /&gt;"The decor is fresh," he said. "It looks new and up-to-date. I had a great time."&lt;br /&gt;The company scrapped players-club cards it had issued for each individual casino in favor of the TrumpONE card, which lets gamblers accrue and redeem perks at any of the casinos, even as it cuts back on those for less profitable gamblers. And it hopes to reduce expenses by $19 million this year.&lt;br /&gt;So, will it all work?&lt;br /&gt;Trump Marina and Trump Plaza regularly rank at or near the bottom of Atlantic City's 11 casinos in terms of monthly revenue. And last month, Trump Entertainment reported that its second-quarter loss widened as gambling revenue fell, partly due to increased regional competition.&lt;br /&gt;But the Taj remains around the top third of Atlantic City casinos in monthly revenue and the casino commission recently granted a new five-year operating licenses for the three Trump casinos despite expressing deep skepticism over the company's rosy financial predictions.&lt;br /&gt;Some analysts say that with land and construction prices pushing the cost of new Atlantic City casinos to the $2 billion range, it might be cheaper to buy an existing one rather than build from scratch. Under such a scenario, the Trump properties might look more attractive.&lt;br /&gt;Earlier this month, KeyBanc Capital Markets cited "excellent" progress on reducing promotional expenses such as freebies for gamblers. And Goldman Sachs wrote in an August report that out-of-state competition is hurting the Trump casinos, but noted that the TrumpONE card program and the Taj expansion should increase revenues.&lt;br /&gt;Researchers found that a half-year exposure to meditation techniques helped patients shave as much as one-third of their psychological distress.&lt;br /&gt;The research team, led by Elizabeth K. Pradhan of the University of Maryland School of Medicine's Center for Integrative Medicine, based its conclusions on an analysis of a specific training course called "Mindfulness-Based Stress Reduction" (MBSR).&lt;br /&gt;Pradhan and her colleagues said the MBSR concept of "mindfulness" is designed to guide patients to focus on the "here and now," while emphasizing the value of calmness, clarity, well-being, and a compassion for oneself and others.&lt;br /&gt;The Arthritis Foundation has said that several so-called "mind-body practices" may help arthritis patients alleviate stress, pain, anxiety and depression.&lt;br /&gt;In addition to meditation, the organization acknowledges the potential of electronic monitoring techniques such as biofeedback; the mental health benefits of guided imagery and hypnosis; and the ability of physical activities such as yoga and tai chi to both calm and energize patients.&lt;br /&gt;The Maryland researchers noted that prior studies had already shown that the MBSR course, in particular, seems to have a positive impact on the psychological symptoms of patients with conditions such as anxiety disorders, chronic pain, fibromyalgia, cancer and multiple sclerosis. Patients recovering from organ transplant surgery also seem to derive some benefit, the researchers said.&lt;br /&gt;For their study, published in the October issue of Arthritis Care &amp;amp; Research, Pradhan and her colleagues focused specifically on rheumatoid arthritis. It was the first-ever analysis of MBSR and its impact on depression, general well-being, and disease progression among rheumatoid arthritis patients, the researchers said.&lt;br /&gt;Thirty-one patients were offered the mediation course over an eight-week period, followed by a four-month maintenance program. Another 32 patients did not participate but were told they would be offered free meditation training once the study was completed.&lt;br /&gt;The consequences of depression can be devastating to the mother, her baby and her entire family, according to the report in the October issue of The American Journal of Psychiatry.&lt;br /&gt;"The prevalence of women diagnosed with depression before, during and after pregnancy was pretty similar," said lead author Patricia Dietz, an epidemiologist at the U.S. Centers for Disease Control and Prevention's Division of Reproductive Health.&lt;br /&gt;SATURDAY, Sept. 29 (HealthDay News) -- U.S. health experts on Friday urged the federal Food and Drug Administration to consider banning the sale of over-the-counter cough and cold medicines for young children.&lt;br /&gt;The recommendation, from FDA safety officials, would apply to decongestant use in children under 2, and antihistamines in those younger than 6, according to agency documents released Friday, the Associated Press reported.&lt;br /&gt;Home equity lines or loans often are touted as a quick and easy way to get out of debt. By leveraging your residence's value, the pitch goes, you can get money to pay off other bills and a tax break, too.&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-2670656402039551357?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/2670656402039551357'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/2670656402039551357'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/equity-home-line.html' title='equity home line'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-4268149630957019793</id><published>2008-10-27T04:42:00.000-07:00</published><updated>2008-10-27T04:43:00.560-07:00</updated><title type='text'>mesothelioma lawsuits</title><content type='html'>Mesothelioma lawsuits require Mesothelioma attorneys experienced in this special type of case. Mesothelioma attorneys are ready and available to assist you with your mesothelioma lawsuit claim. If you have ever been exposed to asbestos and have been diagnosed with mesothelioma, you may be eligible for a mesothelioma lawsuit claim. Contact a LawInfo Lead Counsel qualified mesothelioma attorney today.&lt;br /&gt;As the U.S. Senate considers a bill to protect companies from lawsuits brought by hundreds of thousands of Americans harmed by asbestos, the first-ever analysis of federal mortality records finds that 10,000 Americans die each year from asbestos exposure, and projects that up to ten times that many will die in the next decade.&lt;br /&gt;Although many Americans believe that asbestos has already been banned and its victims have been compensated by the courts, the EWG Action Fund study reports that 30 million pounds of asbestos are used in the U.S. each year, lists dozens of widely-used consumer products that still contain it, and says more than one million workers are exposed every year. Are you one of them?&lt;br /&gt;In 1991, the U.S. Fifth Circuit Court of Appeals overturned what was commonly known as the "Asbestos Ban and Phaseout Rule of 1989." Consequently, scores of asbestos products remain on the market today. The 2001 Mineral Commodity Summary for Asbestos from the U.S. Geological Survey (USGS) reported 61% of the asbestos used in the United States (in 2000) was in roofing products, 19% in friction products, 13% in gaskets, and 7% was classified as "other."&lt;br /&gt;Despite the court's actions, a short list of products still remain banned under the Toxic Substances Control Act (TSCA) and the Clean Air Act. These include: corrugated paper, rollboard, commercial paper, specialty paper, flooring felt, sprayed-on materials containing more than one percent asbestos, and all new asbestos applications are banned.&lt;br /&gt;Mesothelioma Attorneys&lt;br /&gt;Because Mesothelioma is a rare form of cancer that can take up to 20 years to develop, Mesothelioma attorneys need specific training and experience when seeking compensation for clients. Mesothelioma attorneys have a specific team of medical professionals who interpret health records and testing documentation to prove the specific correlation between a patient's side-effects and the Mesothelioma diagnosis.&lt;br /&gt;Once the illness has been properly identified, Mesothelima attorneys must prove that the cause of the illness is the particular exposure to the toxic chemical, at a duration and amount significant enough to cause the health-related symtoms. Often it can take up to 50 years for a person to show the type of dehabilitation necessary for Mesothelioma attorneys to obtain compensation.&lt;br /&gt;Not everyone who works with or was exposed to asbestos can prove that they have suffered injuries related to asbestos contamination. With years of prosecuting the cases that have bankrupted hundreds of asbestos companies, Mesothelioma attorneys have intimate knowledge of the specific evidence necessary to prove the direct correlation between absestos and asbestos-related illnesses such as Mesothelioma and lung cancer. Most recently, WR Grace &amp;amp; Co and its seven executives were charged in conspiring to hide asbestos contamination at a mine in Libby, Montana, which prosecutors claim was responsible for the illnesses of 1200 people resulting in 200 deaths.&lt;br /&gt;Mesothelioma Lawyers&lt;br /&gt;Many Mesothelioma attorneys have asbestos cases ｂａｓｅd on the contamination of vermiculite, a hydrous silicate mineral, shipped by WR Grace to about 240 plants across the country. Federal authorities in 22 states are investigating 28 plants stretching across from North Dakota to Florida and Massachusetts to California, including one plant in Hawaii.&lt;br /&gt;As Mesothelioma attorneys continue to prove the correlation between clients' illnesses and asbestos contimination, legislators struggle to approve a bill that will provide enough compensation for all victims who worked at mining plants and all neighboring residents who were exposed to the toxic chemicals. Currently legislators are having trouble determining how the funding will ensure compensation for victims who will continue to develop asbestos-related illnesses for years to come. The bill has yet to be approved.&lt;br /&gt;Contact a LawInfo Lead Counsel qualified&lt;br /&gt;mesothelioma attorney today.&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-4268149630957019793?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/4268149630957019793'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/4268149630957019793'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/mesothelioma-lawsuits.html' title='mesothelioma lawsuits'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-8167829245463805152</id><published>2008-10-18T01:15:00.000-07:00</published><updated>2008-10-18T01:16:17.496-07:00</updated><title type='text'>The Drum Tower and the Bell Tower (Zhong Gu Lou)</title><content type='html'>The Drum and Bell towers are situated at the northern end of the central axis of the Beijing Inner City to the north of Di' anmen Street.&lt;br /&gt; They are at the heart of one of the most historically picturesque parts of Beijing, surrounded by winding hutongs and situated not far from the northern part of the lakes area.&lt;br /&gt;The Drum Tower was first built in 1272 during the reign of Kublai Khan, at which time it stood at the very heart of the Yuan capital Dadu. At that time it was known as the Tower of Orderly Administration (Qizhenglou).&lt;br /&gt;In 1420, under the Ming Emperor Yongle, the building was reconstructed to the east of the original site and in 1800 under the Qing Emperor Jiaqing, large-scale renovations were carried out. In ancient days, the Drum Tower was the time keeping center for the whole city and was equipped with bronze water clocks and drums that were beaten to mark the hours. The water clocks were later replaced with other timekeeping arrangements. The upper story once housed housed 24 drums, of which only one survives. Its head is made of an entire ox hide and is 1.5 meters in diameter.&lt;br /&gt; A sword slash on the side of the drum was made by soldiers of the Eight-Power Allied Forces during their invasion of Beijing in 1900. In the Qing Dynasty, the hours were marked at night beginning at 7:00 p.m.when the drums were sounded 13 times. After the watch had been "set" in this fashion, each subsequent two-hour interval was marked by a single drum beat. Civil and military officials oriented their lives around these time signals. At the sounding of the third watch (1:00 a.m.) officials attending the morning court audience rose from their beds, and at the fourth (3:00 a.m.) assembled outside the Meridian Gate (Wumen).&lt;br /&gt;At the sounding of the fifth watch (5:00 a.m.) they entered the Imperial Palace and knelt on the Sea of Flagstones (Haimen) before the Hall of Supreme Harmony (Taihedian) to await instructions from the emperor. Close behind the Drum Tower stands the Bell Tower, which first came into use during the reign of the Ming Emperor Yongle. It was destroyed by fire after only a brief existence and it was not until 1747 that Emperor Qianlong undertook its reconstruction. The Bell Tower originally housed a huge iron bell. But because its tolling was not loud enough, this was replaced by a massive cast bronze bell over 10 inches thick that is in perfect condition today. The iron bell was moved to the back of the Drum Tower where it has remained for over 500 years. As recently as 1924, the bronze bell could be heard ringing out the 7:00 p.m. chime from a distance of over 20 kilometers.&lt;br /&gt;According to legend, an official named Deng was given the task of casting the huge bell, and tried unsuccessfully for over a year to carry out the task. As he made one last attempt, his daughter, fearing that her father would be punished if he failed again, decided to sacrifice her life in order to move the gods to bring about a perfect casting, and threw herself into the molten bronze. Her grief-stricken father could only rescue one of her embroidered slippers from the crucible. The casting succeeded and the emperor, moved by the young girl's sacrifice, named her the "Goddess of the Golden Furnace" and built a temple in her honor near the foundry.&lt;br /&gt;After the bell was installed, the chimes could be heard right across the city. But on stormy nights, the bell would emit a desolate moaning sound similar to the word xie, which means "shoe" in Chinese. Recalling the old legend, mothers would comfort their children with: "Go to sleep! The Bell Tower is tolling. The Goddess Who Cast the Bell wants her embroidered slipper back."&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-8167829245463805152?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/8167829245463805152'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/8167829245463805152'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/drum-tower-and-bell-tower-zhong-gu-lou.html' title='The Drum Tower and the Bell Tower (Zhong Gu Lou)'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-6232156927596574336</id><published>2008-10-18T01:12:00.000-07:00</published><updated>2008-10-18T01:13:36.497-07:00</updated><title type='text'>Master in Business Administration</title><content type='html'>Il Master in Business Administration (MBA) è un corso di formazione manageriale post laurea, orientato a fornire ai partecipanti una formazione di general management su tutte le tematiche di gestione di impresa.L'MBA è il più antico fra i corsi universitari post-laurea (dopo i tradizionali 4-5 anni) e come i master in genere è tipico dell'istruzione anglosassone.&lt;br /&gt;L'MBA è quindi il master in materie economiche e gestionali con la più lunga tradizione. La prime Management School e MBA compaiono negli Stati Uniti negli anni '30.Solamente negli ultimi vent'anni sono stati introdotti corsi MBA anche in Italia, come il MIP del Politecnico di Milano, lo SDA dell'Università Bocconi, la Scuola di Management dell'Università Luiss Guido Carli.Tale corso è organizzato dalle maggiori Business School internazionali ed è attualmente considerato come il più alto grado di specializzazione che si possa acquisire in questo campo.&lt;br /&gt;Alcune aziende ed organizzazioni internazionali pagano l'MBA ad alcuni tra i loro migliori dipendenti, sia durante la loro attività lavorativa (MBA part-time) sia durante una pausa (con un contratto di riassunzione finito il corso). In un caso, il dipendente frequenta un master serale oppure assentandosi alcune ore retribuite alla settimana durante l'orario di lavoro; nell'altro caso, utilizza il cosiddetto "anno sabbatico", un anno non retribuito che il lavoratore può chiedere una volta nella vita lavorativa per fare qualcosa che ritiene importante per sé, e che non è tenuto a giustificare, come viaggi, una laurea per cultura personale o qualcosa di utile per la propria carriera.Si tratta di un investimento, in particolare per le aziende che decidono di crescere per linee interne, senza "importare" i dirigenti da imprese che operano in settori o con prodotti completamente differenti. Dato l'importo elevato delle rette per un MBA, l&lt;br /&gt;'azienda richiede al dipendente un tempo di 1-2 anni di permanenza minima in azienda (come dirigente) a conclusione del master per recuperare l'investimento fatto sulla persona. Se il dipendente cerca una migliore posizione lavorativa altrove, è tenuto a risarcire all'impresa parte o tutto l'importo sostenuto.Molti manager di grandi aziende&lt;br /&gt; internazionali hanno conseguito un MBA presso una delle business school: il diploma MBA è considerato un simbolo di successo e una garanzia di una carriera lavorativa più che brillante.I corsi sono focalizzati sia sulle aree strettamente tecniche (principalmente finanza, marketing ed economia) sia sullo sviluppo delle doti personali (come leadership e management) richieste nel mondo corporativo; in generale però il programma&lt;br /&gt; varia da scuola a scuola anche secondo il tipo di corso prescelto, ma sicuramente parte dell'apprendimento avviene sempre tramite l'analisi di casi pratici e innumerevoli attività di gruppo.Presso le principali scuole di management sono sorte associazioni di ex alunni MBA, che favoriscono lo sviluppo di relazioni culturali e professionali tra i diplomati. Tra le più note c'è l'AMBA (Bocconi, Milano), l'Assomaster (Luiss, Roma), l'AMIP (Politecnico, Milano).&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-6232156927596574336?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/6232156927596574336'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/6232156927596574336'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/master-in-business-administration.html' title='Master in Business Administration'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-897894696770005772</id><published>2008-10-18T01:09:00.002-07:00</published><updated>2008-10-18T01:11:03.874-07:00</updated><title type='text'>To say that it was DNA-based is supposition</title><content type='html'>As I pointed out above, 3-4 billion years ago life may have been based on RNA.&lt;br /&gt;To say that it was DNA-based is supposition. If we have no references to support a statement, then it has to be removed as it is not attributed to a reliable source. If you can find a reference that says when DNA evolved, then I will be happy to add that date.&lt;br /&gt;I have been unable to find any such references. TimVickers 05:15, 11 March 2007 (UTC) The point is made well in the wiki. The presence of a hydroxyl group at the 2'-position of the ribose sugar is what separates our positions. Because of the weaker information transmission vector of RNA it seems that DNA was a stong contender from the start.&lt;br /&gt; Lumping them together and dating them that way holds the best path forward. I think the wiki reads well as it has evolved. The time line link is very good and more than covers the point I was making. Thank you for your time. RoddyYoung 13:45, 12 March 2007 (UTC)[edit] Suggested editsOverall the article is excellent, congratulations.&lt;br /&gt;I noted some issues in a few places. See what you think:DNA p. 2 What is meant by "act directly"? Distinguish binding from catalysis. Reworded. p. 3 Implies that a given RNA can be used either as message or structural RNA. Reworded. Physical and chemical properties p. 1 The width is 3.3 angstroms. From the reference "Combined measurement of the rotational and translational frictional coefficients of rod-like DNA molecules in dilute aqueous solution yields 22 to 26 Å for the hydrodynamic diameter and 3·34(± 0·1)&lt;br /&gt;Å for the length per base-pair."p. 3 third and fifth carbon atoms in adjacent sugar rings. Reworded. p. 4 When does uracil not replace thymine in RNA? I have to check my references, but when the 5 position of uracil is modified, it is typically more elaborate than simple methylation, e.g., 5-methyl-amino-methyl-2-thio-uridine. Ribosylthymine is found in some RNAs such as tRNAs, added ref. p. 5 These grooves result from the relative position of the glycosidic bonds for a base pair, as seen in the figure. Indeed, feel free to add this if you think this would make the section clearer. Sense and antisense p. 1 The 5' to 3' copying is irrelevant to sense and antisense. It results from the nature of the substrates.&lt;br /&gt;Good point, removed. Supercoiling The basic explanation is inaccurate. Although there may be some distortion at the level of the double helix, the main effect is to cause intertwining of different parts of the double helix, hence, super-coiling. I disagree, the main effect in biology is to alter the ease with which the strands can be separated. DNA damage Double strand breaks are dangerous because there is no easy way to fix them in non-replicating cells. Telliott 21:18, 15 February 2007 (UTC) Expanded. Thank you for a most though review. TimVickers 23:06, 15 February 2007 (UTC) Base StackingThis article erroneously states that the two strands of DNA are held together by H-bonds between bases on the antiparallel strands, when in fact it is more base stacking that contributes to the stability of DNA. H-bonds certainly play a part, but certainly not as much as the stacking does. I would be glad to add the edit, but unfortunately the article is still semi-protected. [3] --Redsquareblack 18:26, 24 February 2007 (UTC)Reworded the sentence to The DNA double helix is stabilized by hydrogen bonds between the bases attached to the two strands. Base stacking is discussed further in the base pairing section.. This arrangement of two nucleotides joined together across the double helix is called a base pair. In a double helix, the two strands are also held together by forces generated by the hydrophobic effect and pi stacking, but these forces are not affected by the sequence of the DNA.[14] As you are logged in, Redsquareblack, you can edit semi-protected pages. This only blocks anonymous IP editing. TimVickers 19:15, 24 February 2007 (UTC)&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-897894696770005772?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/897894696770005772'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/897894696770005772'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/to-say-that-it-was-dna-based-is.html' title='To say that it was DNA-based is supposition'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-2213941184407589591</id><published>2008-10-18T01:09:00.001-07:00</published><updated>2008-10-18T01:09:32.089-07:00</updated><title type='text'>The AGE OF DNA</title><content type='html'>reproduction, a process in biological DNA that dates back 3.5 - 4.6 billion years.[1]I have removed this as it is inaccurate, early life may not have used DNA as its genome, RNA is a possibility but we have no evidence. Similarly, we have no evidence when sexual reproduction evolved. Most of the history of life on earth is bacterial and archaeal, and these organisms reproduce asexually.&lt;br /&gt;TimVickers 17:07, 14 February 2007 (UTC)I have added some material on the evolutionary history of DNA to the "Overview of biological functions" introduction. TimVickers 17:54, 14 February 2007 (UTC) Hi Tim this is interesting that time frames of 3 billion years plus or minus a billion are not able to be included.&lt;br /&gt;DNA needs a structure of time to place life, sexual life, and asexual life into. I would suggest at this point that the time line of atomic formulation is important to discuss. All heavy elements like Gold and Uranium are formed in exploding stars or supernova and then recollected together to from solar systems. The atoms that form molecules that then form DNA have time lines that take place after atom formulation and conglomeration. As we look back into time we can say that DNA formed between the formation of Atoms/Molecules and say up to the formation of the Wikipedia page on DNA.&lt;br /&gt;This is a large time period and we know something happened in there. I give you your point that it is hard to prove but I still suggest that a time frame is important at the beginning of this page for new readers to DNA to have some perspective on when DNA started the formation of life. First the solar system formed, then DNA formed and then Wikipedia formed. Please puts some dates on this to inform the reader.RoddyYoung 09:23, 24 February 2007 (UTC)The formation of atoms is indeed a very interesting area. However, a problem I see in adding information to this article on the formation of matter is that this material would have to be repeated in every article that deals with mater-based objects.&lt;br /&gt;It might be better to simply add this once to the specific article on matter. TimVickers 17:10, 24 February 2007 (UTC) The same agument you put forward on matter could be used for adding a time frame as to when DNA and RNA started replicating. I agree with you that Matter formation does not have to be included in the DNA page due to its generally accepted understanding. However to not include a date for the formation for DNA in the DNA page is missing an important element in the wiki. RoddyYoung 13:16, 6 March 2007 (UTC) If you can find a reliable high-quality source that deals with this, then please by all means add it to the section on "Evolution of DNA-based metabolism".&lt;br /&gt;However, I have not been able to find this information, since, as I explain in this section, I don't think anybody really knows. TimVickers 17:18, 6 March 2007 (UTC) The development of DNA by Stephen Hawking is outlined here and covers the points I made as well as your points. This will give the reader a background. However after you read it could you put a date on when you understand DNA to have formed 12 billion years as an outside (when no carbon atoms existed in the universe) to 2001 when wikipedia was invented and DNA could be edited in this form. If you can narrow this down form my inadequate attempt then that would be good. Other wise I am happy for my numbers to stand and go into the main document.RoddyYoung 04:48, 11 March 2007 (UTC)In that document it states "&lt;br /&gt;We do not know how DNA molecules first appeared." It also does not state when this might have occurred. I don't see how I can use that document to write about how and when DNA originated. TimVickers 15:14, 10 March 2007 (UTC) "We do not know how DNA molecules first appeared" does not imply that they did not appear. (Truism). Dating when DNA appeared is independant as to how DNA molecules first appeared. We see today how DNA works and we know also that the complimentary stand process was the start of the process back then and that complimentary stand replication links all the way through time to today.&lt;br /&gt; I am making these points as I was happy with the time frame 4 billion to 3 billion years ago DNA formed and replicated. By deleting this you become the gate keeper of the timing and by providing no date you perport that is better than a date plus or minus a billion years. You should not be able to have it both ways. RoddyYoung 04:48, 11 March 2007 (UTC)&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-2213941184407589591?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/2213941184407589591'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/2213941184407589591'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/age-of-dna.html' title='The AGE OF DNA'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-2322197122307913588</id><published>2008-10-18T01:07:00.000-07:00</published><updated>2008-10-18T01:08:43.695-07:00</updated><title type='text'>Looking at other news sources</title><content type='html'>Looking at other news sources, a search for DNA in Google news gives 24,488 articles, while "deoxyribonucleic acid" gives just 51. Tim Vickers (talk) 22:02, 19 November 2007 (UTC) I gotta go with the status quo here.&lt;br /&gt;I work in a large research institute on projects that emphasize cell and molecular biology (I'm being vague to protect my privacy), and I can't remember the last time that I or any of my colleagues used the term deoxyribonucleic acid. Yes, DNA stands for deoxyribonucleic acid, but even among experts in the field nobody uses the full form. Is there any harm in having DNA under the name DNA? TenOfAllTrades(talk) 22:39, 19 November 2007 (UTC) There are several benefits in having DNA under its full name: technical accuracy, clarity of information, consistency in style, etc. The guideline is very clear: "&lt;br /&gt;Acronyms can be used in page naming if the term you are naming is almost exclusively known only by its acronyms and is widely known and used in that form." I have done my duty; I've expressed my doubt whether this is true and have even supported it with a poll. Now it's up to you to a) prove otherwise, b) explain why this guideline does not apply here (while at the same time it obviously does for LSD,&lt;br /&gt;THC, GABA, ISDN, ISBN etc), or c) gain consensus and change its wording. We're writing an encyclopedia, which means we should not use jargon like it is used in news and elsewhere unless DNA is indeed almost exclusively known by its acronym. --Eleassar my talk 09:20, 20 November 2007 (UTC)It is exclusively known by its acronym. David D. (Talk) 14:08, 20 November 2007 (UTC) This suggestion has been brought up before, what would people think about a straw poll to try to get a better idea of the balance of views on this issue? Tim Vickers (talk) 19:11, 20 November 2007 (UTC)I think it is a waste of time. The basis of this argument is that LSD is at its full name but a reading of the wikipedia guidelines is clear that: "Acronyms can be used in page naming if the term you are naming is almost exclusively known only by its acronyms and is widely known and used in that form."&lt;br /&gt; If DNA is not an example of this exception then what is? Far better would be a discussion of why Lysergic acid diethylamide is not at LSD rather than using that as a precedent to move this article to deoxyribonucleic acid. David D. (Talk) 19:39, 20 November 2007 (UTC) My straw vote would go to “DNA”. Although it’s possible to use the full name in the article title, it seems silly if during the rest of the article it will be referred to only as DNA (not just because it’s shorter, but because it’s less confusing). And as a scientist, “deoxyribonucleic acid” falls under the category of “trivia question” rather than “practical term”, at least for me. Dihydrogen monoxide anyone? (sorry, couldn’t resist...)&lt;br /&gt;Forluvoft (talk) 20:14, 20 November 2007 (UTC) Even searching in the scientific database PubMed gives you 938,211 results for DNA, and only 10,567 for "deoxyribonucleic acid" - almost a 100:1 ratio. Tim Vickers (talk) 20:35, 20 November 2007 (UTC) I've proposed Lysergic acid diethylamide to be moved to LSD. You may comment this at Talk:Lysergic acid diethylamide. --Eleassar my talk 12:30, 4 December 2007 (UTC)&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-2322197122307913588?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/2322197122307913588'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/2322197122307913588'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/looking-at-other-news-sources.html' title='Looking at other news sources'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-8003852600523386796</id><published>2008-10-18T01:04:00.001-07:00</published><updated>2008-10-18T01:06:53.288-07:00</updated><title type='text'>Title: DNA or deoxyribonucleic acid?</title><content type='html'>IMO the article should be moved to deoxyribonucleic acid. I find it very strange to use acronym here while at the same time we have LSD under Lysergic acid diethylamide. The word deoxyribonucleic acid explains the structure of the molecule and the people who don't know what DNA stands for will be redirected to deoxyribonucleic acid. --Eleassar my talk 11:19, 19 November 2007 (UTC)99.95% of our readers will search for DNA,&lt;br /&gt;so that's the title we should use - see the Wikipedia:Naming conventions which states: "Generally, article naming should prefer what the majority of English speakers would most easily recognize, with a reasonable minimum of ambiguity, while at the same time making linking to those articles easy and second nature." see also Talk:DNA/Archive 6 for last time this was discussed. Tim Vickers (talk) 16:47, 19 November 2007 (UTC)&lt;br /&gt; From the same page: "Avoid the use of abbreviations, including acronyms, in page naming unless the term you are naming is almost exclusively known only by its abbreviation and is widely known and used in that form.". I don't think this is the case here. --Eleassar my talk 19:29, 19 November 2007 (UTC)I disagree, DNA is almost exclusively known by its abbreviation and is certainly most widely known and used in this form. Ask 100 people on the street what DNA stands for, how many do you think will be able to give you the correct answer? Tim Vickers (talk) 19:33, 19 November 2007 (UTC)&lt;br /&gt; I agree with Tim, I'm betting most people don't even know its an acronym. David D. (Talk) 19:53, 19 November 2007 (UTC) And only 0.0001% of people will know if "deoxyribo" or "deoxyribose" is correct. I know I had to check! :) Tim Vickers (talk) 20:05, 19 November 2007 (UTC) This surprises me very much. I was sure the majority of people know "DNA" means "deoxyribonucleic acid". I'll really do the test you have proposed. :) --Eleassar my talk 20:15, 19 November 2007 (UTC)Be sure to ask if it is "ribo" or "ribose"! Tim Vickers (talk) 20:49, 19 November 2007 (UTC) Ok, according to this pool two thirds of Americans know what does DNA stand for. This clearly refutes your claim that DNA is&lt;br /&gt; almost exclusively known only by its initials. Can you prove otherwise? --Eleassar my talk 21:40, 19 November 2007 (UTC)Untrue, in a multiple choice format 2/3 of people could select the correct answer, we don't know what the alternatives were or how many alternatives were given. Most obviously, choosing something from a list isn't the same as using the term in common discourse or even being able to recall it by&lt;br /&gt;oneself. That news article itself uses the term DNA throughout, even in its headline - it doesn't say "The deoxyribonucleic acid IQ of americans! Tim Vickers (talk) 21:45, 19 November 2007 (UTC)&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-8003852600523386796?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/8003852600523386796'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/8003852600523386796'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/title-dna-or-deoxyribonucleic-acid_18.html' title='Title: DNA or deoxyribonucleic acid?'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-2576811477467617726</id><published>2008-10-18T01:04:00.000-07:00</published><updated>2008-10-18T01:06:14.507-07:00</updated><title type='text'>Title: DNA or deoxyribonucleic acid?</title><content type='html'>IMO the article should be moved to deoxyribonucleic acid. I find it very strange to use acronym here while at the same time we have LSD under Lysergic acid diethylamide.&lt;br /&gt;The word deoxyribonucleic acid explains the structure of the molecule and the people who don't know what DNA stands for will be redirected to deoxyribonucleic acid. --Eleassar my talk 11:19, 19 November 2007 (UTC)99.95% of our readers will search for DNA, so that's the title we should use - see the Wikipedia:Naming conventions which states: "Generally, article naming should prefer what the majority of English speakers would most easily recognize, with a reasonable minimum of ambiguity, while at the same time making linking to those articles easy and second nature." see also Talk:DNA/Archive 6 for last time this was discussed. Tim Vickers (talk) 16:47, 19 November 2007 (UTC) From the same page: "&lt;br /&gt;Avoid the use of abbreviations, including acronyms, in page naming unless the term you are naming is almost exclusively known only by its abbreviation and is widely known and used in that form.". I don't think this is the case here. --Eleassar my talk 19:29, 19 November 2007 (UTC)I disagree,&lt;br /&gt;DNA is almost exclusively known by its abbreviation and is certainly most widely known and used in this form. Ask 100 people on the street what DNA stands for, how many do you think will be able to give you the correct answer? Tim Vickers (talk) 19:33, 19 November 2007 (UTC) I agree with Tim, I'm betting most people don't even know its an acronym. David D. (Talk) 19:53, 19 November 2007 (UTC) And only 0.0001% of people will know if "deoxyribo" or "deoxyribose" is correct. I know I had to check! :) Tim Vickers (talk) 20:05, 19 November 2007 (UTC) This surprises me very much. I was sure the majority of people know "DNA" means "deoxyribonucleic acid".&lt;br /&gt;I'll really do the test you have proposed. :) --Eleassar my talk 20:15, 19 November 2007 (UTC)Be sure to ask if it is "ribo" or "ribose"! Tim Vickers (talk) 20:49, 19 November 2007 (UTC) Ok, according to this pool two thirds of Americans know what does DNA stand for. This clearly refutes your claim that DNA is almost exclusively known only by its initials. Can you prove otherwise? --Eleassar my talk 21:40, 19 November 2007 (UTC)Untrue, in a multiple choice format 2/3 of people could select the correct answer, we don't know what the alternatives were or how many alternatives were given.&lt;br /&gt;Most obviously, choosing something from a list isn't the same as using the term in common discourse or even being able to recall it by oneself. That news article itself uses the term DNA throughout, even in its headline - it doesn't say "The deoxyribonucleic acid IQ of americans! Tim Vickers (talk) 21:45, 19 November 2007 (UTC)&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-2576811477467617726?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/2576811477467617726'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/2576811477467617726'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/title-dna-or-deoxyribonucleic-acid.html' title='Title: DNA or deoxyribonucleic acid?'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-2298432425230839289</id><published>2008-10-18T01:03:00.001-07:00</published><updated>2008-10-18T01:04:52.210-07:00</updated><title type='text'>Brief description</title><content type='html'>Changsha sits at the northeast of Hunan Province in south-central China, fed by the Xiang River, west of Jiangxi and south of Hubei. It is known for its warm climate and is the economic and political capital of Hunan. Thanks to its position as the central transport hub for Hunan, it is also easily reached by air, rail and road, with a limited passenger service bringing visitors by water. Changsha's lush farmland was for years the core of its economic success, but in the middle of the 19th century, it went through a period of modernization and today is an important economic center with particular strengths in construction machinery manufacturing, electronics and IT and food processing.&lt;br /&gt; Cities all across China celebrate a wide range of festivals throughout the year and Changsha is no exception. The Dragon Boat Festivals are celebrated nationally to mark the ritual suicide in 278 BC of the poet Qu Yuan, who was protesting against official corruption. The boat races symbolize the people's desperate attempts to save him and recover his body: the local significance of the ceremonies derives from the fact that Qu Yuan died in the Miluo River, not far north of the city.&lt;br /&gt;The Forest Protection Festival is unique to the city, promoting and rejoicing in the fine natural resources in Zhangjiajie Park. The China Liuyang International Fireworks Festival, which marks the Hunan Province as one of the earliest regions to produce fireworks, is perhaps a more lively time to visit the city, when long firework displays are put on to celebrate this historical connection. By far the most exciting historical attraction at Changsha is the site of the Mawangdui Han Dynasty Tombs outside the city which were discovered in the 1970s. They contained a treasure trove of Han Dynasty artifacts, as well as three well-preserved mummies. The history of Changsha can be further explored at the Hunan Provincial Museum where Shang and Zhou bronze vessels sit alongside a collection of Yuezhou celadon porcelain and an original text of the legendary calligrapher Wang Xizhi's most famous work "Prologue to the Orchid Pavilion Collection".&lt;br /&gt;Visitors can also drop in on the Changsha Hunan Xiangxiu Museum and the Hunan Museum of Geology which holds collections of dinosaur bones found in the Hunan province and a planetarium. Changsha has a firm place in the modern history of China, as Chairman Mao – the Father of modern China – grew up in the nearby village of Shaoshan. His childhood home is now known as Chairman Mao Zedong's Former Residence and receives visitors daily. Within the city, his former office and another residence can also be found by those interested in understanding the birth of Chinese Communism.&lt;br /&gt;Changsha Museum has a collection calligraphy and prose written in Chairman Mao's calligraphy. Yuelu Academy is the second of the most popular sights in Changsha, having been established on the slopes of Yuelu Mountain during the Song Dynasty to educate those intending to take China's rigorous Civil Examinations and receive a classical education. It is still a thriving institute of Higher Education. Yuelu Mountain itself is known as a wonderful spot to visit, dotted with sites including the Qin Dynasty Yuelu Palace and a number of Qin Dynasty tombs including the Huangxin Tomb, the Cai'e Tomb and the Chentianhua Tomb. Hengshan Mountain just outside of Changsha home to a number of Taoist and Buddhist temples including the Nanyue Da Miao which is the largest temple in southern China. The wonderfully fragrant Orange Isle in Xiang River on which sits the Heavenly Heart Pavilion located on is another beautiful location where the views over the city are considered particularly fine.&lt;br /&gt;Changsha is a seat of Hunan Cuisine, one of the eight regional cuisines of China characterized by hot spicy flavors, fresh aromas and the use of color. Visitors may like to hunt out Hong Shao Rou, a braised pork dish which melts in the mouth and was known to be Chairman Mao's favorite. Location /Climate Changsha is located in the Hunan Province on the lowest part of the Xiang River. It is humid all year round, with a summer average maximum temperature of around 33C (91.4F). The coldest month of January has average minimums of 1C (33F). Area &amp;amp; Population The area of Changsha covers 10% of the Hunan Province: an area of 11,819 square kilometers. The urban area of Changsha is relatively small in comparison, comprising an area of 554 sq. kilometers. The population of Changsha is just under 6.4 million, with an urban population of 2.09 million. History There has been a settlement in the area now known as Changsha since the Neolithic era. It was a fortified city by the second century BC and grew further during the Han Dynasty when it became the capital of the Changsha Kingdom. During the Han Dynasty, the famous Mawangdui Tombs were constructed to house the remains of the Marquis of Dai, his wife and a second male who archeologists believe was their son.&lt;br /&gt;A number of finds in the tomb have helped piece together Changsha's historical importance: rare volumes written on silk were found in the tombs along with a large collection wooden figurines which have provided valuable links between costume and status in Western Han society. Yuelu Academy was founded during the Song Dynasty in Changsha and evolved over the next 1000 years into a modern higher education institution. Zhu Xi and Zhang Shi the renowned Confucian Scholars both lectured at the academy. As a part of the Treaty of Shanghai in 1903, Changsha was opened up to foreign trade, as a consequence of which the city experienced a boom when factories, churches and schools were built with the resultant influx of money and people. Yale University alumni opened a college in the city, which later became the Xiangya Medical Centre and the Yali Secondary School.A large part of the history of Changshan and the area around it is based on its connection with Chairman Mao.&lt;br /&gt; He was brought up in the small village of Shaoshan situated about 100 kilometers outside of Changshan, later attending Hunan Number 1 Teachers' Training School in the city. Two of his former residences and his former office are now open to visitors. Four large battles took place in Changsha during the Second Sino-Japanese War, when invading Japanese forces tried to capture it from the Chinese Nationalists who had secured the area. The 4th Battle of Changsha ended when the city finally fell to Japanese forces who held it for a year before being defeated in a counter-attack. The city became part of the territory of the People's Republic of China in 1949. 　&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-2298432425230839289?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/2298432425230839289'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/2298432425230839289'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/brief-description_18.html' title='Brief description'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-8357561678199339647</id><published>2008-10-18T01:03:00.000-07:00</published><updated>2008-10-18T01:04:47.383-07:00</updated><title type='text'>Brief description</title><content type='html'>Changsha sits at the northeast of Hunan Province in south-central China, fed by the Xiang River, west of Jiangxi and south of Hubei. It is known for its warm climate and is the economic and political capital of Hunan. Thanks to its position as the central transport hub for Hunan, it is also easily reached by air, rail and road, with a limited passenger service bringing visitors by water. Changsha's lush farmland was for years the core of its economic success, but in the middle of the 19th century, it went through a period of modernization and today is an important economic center with particular strengths in construction machinery manufacturing, electronics and IT and food processing.&lt;br /&gt; Cities all across China celebrate a wide range of festivals throughout the year and Changsha is no exception. The Dragon Boat Festivals are celebrated nationally to mark the ritual suicide in 278 BC of the poet Qu Yuan, who was protesting against official corruption. The boat races symbolize the people's desperate attempts to save him and recover his body: the local significance of the ceremonies derives from the fact that Qu Yuan died in the Miluo River, not far north of the city.&lt;br /&gt;The Forest Protection Festival is unique to the city, promoting and rejoicing in the fine natural resources in Zhangjiajie Park. The China Liuyang International Fireworks Festival, which marks the Hunan Province as one of the earliest regions to produce fireworks, is perhaps a more lively time to visit the city, when long firework displays are put on to celebrate this historical connection. By far the most exciting historical attraction at Changsha is the site of the Mawangdui Han Dynasty Tombs outside the city which were discovered in the 1970s. They contained a treasure trove of Han Dynasty artifacts, as well as three well-preserved mummies. The history of Changsha can be further explored at the Hunan Provincial Museum where Shang and Zhou bronze vessels sit alongside a collection of Yuezhou celadon porcelain and an original text of the legendary calligrapher Wang Xizhi's most famous work "Prologue to the Orchid Pavilion Collection".&lt;br /&gt;Visitors can also drop in on the Changsha Hunan Xiangxiu Museum and the Hunan Museum of Geology which holds collections of dinosaur bones found in the Hunan province and a planetarium. Changsha has a firm place in the modern history of China, as Chairman Mao – the Father of modern China – grew up in the nearby village of Shaoshan. His childhood home is now known as Chairman Mao Zedong's Former Residence and receives visitors daily. Within the city, his former office and another residence can also be found by those interested in understanding the birth of Chinese Communism.&lt;br /&gt;Changsha Museum has a collection calligraphy and prose written in Chairman Mao's calligraphy. Yuelu Academy is the second of the most popular sights in Changsha, having been established on the slopes of Yuelu Mountain during the Song Dynasty to educate those intending to take China's rigorous Civil Examinations and receive a classical education. It is still a thriving institute of Higher Education. Yuelu Mountain itself is known as a wonderful spot to visit, dotted with sites including the Qin Dynasty Yuelu Palace and a number of Qin Dynasty tombs including the Huangxin Tomb, the Cai'e Tomb and the Chentianhua Tomb. Hengshan Mountain just outside of Changsha home to a number of Taoist and Buddhist temples including the Nanyue Da Miao which is the largest temple in southern China. The wonderfully fragrant Orange Isle in Xiang River on which sits the Heavenly Heart Pavilion located on is another beautiful location where the views over the city are considered particularly fine.&lt;br /&gt;Changsha is a seat of Hunan Cuisine, one of the eight regional cuisines of China characterized by hot spicy flavors, fresh aromas and the use of color. Visitors may like to hunt out Hong Shao Rou, a braised pork dish which melts in the mouth and was known to be Chairman Mao's favorite. Location /Climate Changsha is located in the Hunan Province on the lowest part of the Xiang River. It is humid all year round, with a summer average maximum temperature of around 33C (91.4F). The coldest month of January has average minimums of 1C (33F). Area &amp;amp; Population The area of Changsha covers 10% of the Hunan Province: an area of 11,819 square kilometers. The urban area of Changsha is relatively small in comparison, comprising an area of 554 sq. kilometers. The population of Changsha is just under 6.4 million, with an urban population of 2.09 million. History There has been a settlement in the area now known as Changsha since the Neolithic era. It was a fortified city by the second century BC and grew further during the Han Dynasty when it became the capital of the Changsha Kingdom. During the Han Dynasty, the famous Mawangdui Tombs were constructed to house the remains of the Marquis of Dai, his wife and a second male who archeologists believe was their son.&lt;br /&gt;A number of finds in the tomb have helped piece together Changsha's historical importance: rare volumes written on silk were found in the tombs along with a large collection wooden figurines which have provided valuable links between costume and status in Western Han society. Yuelu Academy was founded during the Song Dynasty in Changsha and evolved over the next 1000 years into a modern higher education institution. Zhu Xi and Zhang Shi the renowned Confucian Scholars both lectured at the academy. As a part of the Treaty of Shanghai in 1903, Changsha was opened up to foreign trade, as a consequence of which the city experienced a boom when factories, churches and schools were built with the resultant influx of money and people. Yale University alumni opened a college in the city, which later became the Xiangya Medical Centre and the Yali Secondary School.A large part of the history of Changshan and the area around it is based on its connection with Chairman Mao.&lt;br /&gt; He was brought up in the small village of Shaoshan situated about 100 kilometers outside of Changshan, later attending Hunan Number 1 Teachers' Training School in the city. Two of his former residences and his former office are now open to visitors. Four large battles took place in Changsha during the Second Sino-Japanese War, when invading Japanese forces tried to capture it from the Chinese Nationalists who had secured the area. The 4th Battle of Changsha ended when the city finally fell to Japanese forces who held it for a year before being defeated in a counter-attack. The city became part of the territory of the People's Republic of China in 1949. 　&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-8357561678199339647?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/8357561678199339647'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/8357561678199339647'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/brief-description.html' title='Brief description'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-1820452690933289533</id><published>2008-10-18T01:00:00.002-07:00</published><updated>2008-10-18T01:03:32.344-07:00</updated><title type='text'>London 8 minutes</title><content type='html'>　　The dancing was a bit straggly in the " London " routine . Afterseeing all the cyclists taking part,the Chinese will think "Nobodycan afford cars in London "&lt;br /&gt;　　The British "teaser" was ok, but we were disappointed to seethe performer in the wheelchair was not in fact disabled but wasleaping around later in the dance.&lt;br /&gt;　　Did anyone else this the 'London 8 minutes' presentation was -dare I say it - rubbish!? Leona sang her heart out on top of aladder, on top of a bus whilst 20 contemporary dancers did theirstuff way below. It was just so low key relative to what had goneon beforeit.　&lt;br /&gt;　But oh, our offering at the closing ceremony was embarrassing.Ared bus,a pop singer, an ageing rocker and an overpaid ball kickerwas awful. Where was our great Island heritage portrayed in allthis flummery?Organisers - please do betterfor us in 2012.&lt;br /&gt; 　 And why can't Boris button his jacket? Too fat or badtailoring?&lt;br /&gt;　 I've just watched the Olympic closing ceremony, and as I feared,Britain's contribution was embarassing and totally pathetic - Ijust hope they manage to do better in 2012. It will obviously beimpossible to rival the spectacular pageant that Beijing provided,but I thought the dancing (if you can call that dancing) and theLeona Lewis/Jimmy Page effort was just pitiful. Hopefully, we canditch all this government inspired 'Cool Britannia' garbage andpresent something a bit more slick and professional.&lt;br /&gt;　　I agree that Adrian Childs was poor: he seemed to think that hewas on the One Show.　　　　The London Bus routine was pretty much rubbish ... the music waspoor and everything smacked of pop culture. Period.&lt;br /&gt;　　　The Games themselves were superbly run. The BBC was teetering onthe brink of overblown nationalism, however.&lt;br /&gt;　　Jimmy Page and David Beckham have been massive icons of Britishpopular culture. I'm not especially a fan of either, but theyperfectly suited to the ceremony.&lt;br /&gt;　　　　Not bothered either way about Leona whatshername, but the chiefembarrassment, reducing us instantly to laughing stock of the worldand diminishing at a stroke the achievements of our athletes, wasthe presence of Boris Bloody Johnson!!! This man symbolizeseverything wrong with Britain - a deeply untalented buffoon, with athoroughly sinister interior. Get him off the stage once and forall.&lt;br /&gt;　　When I heard about the plans for the London presentation,including the "arrangement" of the national anthem and the rehashed70s rock music, I thought "it can't possibly be as bad as I'mimagining it". Well, it wasn't. It was a thousand, toe-curlinglyhideous times worse. Against the dignity, seriousness and power ofthe Chinese displays, it was so far beyond embarrassing, my brainhas already almost forgotten it, in a desperate paroxysm ofself-defence.　omg, I hope our eight minutes during the closing ceremony is nota foretaste of the tackiness to come in four years time. "&lt;br /&gt;　　　　It felt a lot longer than eight minutes..... Every cringe andevery gasp of horror made it feel like a lifetime!　&lt;br /&gt;　　　If that is all we have to offer, lets hope the rest of the worldhas popped out down the pub when we take centre stage.&lt;br /&gt;　　　　Our athletes deserve much better than this, they did us proud inBeijing (lets not forget the Paralympics too) and will continue todo so.&lt;br /&gt;　　Mind you, from the wretched logo to the vacuous miming chick toDavid Bloody Beckham, it was all a perfect reflection of London andEngland as they now are: cheap, tatty, shallow, and above alllaughing enormously at their own jokes while the rest of the worldlooks on with contempt. Perfect. 　&lt;br /&gt;　　　Now, 2012... where can I go to avoid it all? Please can I move toChina?　&lt;br /&gt;　I have been glued to the Olympics constantly and very proud ofTeam GB but my god what on earth was that 8 minute section in theclosing ceremony.　　&lt;br /&gt;　　The chinese put on a fantastic show and we have basically a redbus and a sparkler.　　　　Who ever put this together should be sacked before we areembarressed anymore.　　&lt;br /&gt;　　By the way BBC should be given a gold medal for the coverage,first class.&lt;br /&gt;　　I have a Grandson living and working in Beijing and I only hopehe stays under cover until the humilation dies down a little. Itcertainly will never go away.&lt;br /&gt;　　 　　In 8 mins we have become the laughing stock of the world.　&lt;br /&gt;　　—— Leona！ Poor girls must be so nervous!　　—— She sounds like a whale mating 　　　　　　I didn't think it was possibly but this both sucks and blows.&lt;br /&gt;　　　　　Grow up London 2012 and stop trying to be so 'cool'. This isembarrassing.&lt;br /&gt;　　The Chinese did it again the closing was amazing. First class andout of this world. The one negative was the London Show what in theheck was that. In Athens when the Chinese did there welccome toshow it was amazing and you knew you where going to get somethingspecial in Beijing. I hope that London does better than what theyshowed to-day.&lt;br /&gt;　　Replicate it, we don’t have a chance it hell. For one thing we’llbe lucky to find 1% of the volunteers from Beijing. Perhaps all thepeople claiming on benefits should be forced to volunteer!!!　　&lt;br /&gt;　　Smith, London　　Forget about the image of Myra Hindley. What about the image ofsome guy painting graffiti on a wall? What are we trying to sayhere? "Come to London, where criminal disfigurement of publicproperty is welcomed and celebrated?!"　&lt;br /&gt;　 　　Dont know what to laugh more at - the tacky 8 minutes of rubbishthe UK put on in Beijing or the smug comments on here about how "Atleast we are free.." Last time I looked the UK was the most watched(CCTV) nation on earth and we have a government that has used fearand hate to strip us of freedoms on a daily basis. The onlydifference between the UK and China is that they wasted billionsmore than we can afford to- but Im sure we'll waste as much as wecan. But freedom?? Dont make me laugh!　　&lt;br /&gt;　　Terry Roberts, Shropshire　&lt;br /&gt;　The Chinese people seem to love all things western (David Beckhamincluded by the looks of it) but in return all we can do is badmouth them and show no respect. What does that say aboutus.　　　　There’s an old saying, treat people the same as how you wouldlike to be treated.　&lt;br /&gt;　Oops, I forgot about the fireworks. Weren't theywonderful!　&lt;br /&gt;　　　I was so impressed by how they managed to create the OlympicRings in the sky - truly amazing.　　&lt;br /&gt;　　Thank you China.　　　&lt;br /&gt;　In my time I've witnessed countless Royal Variety Performancesand the opening ceremony of the Dome. This British fiasco was worsethan any of them, and even at only eight minutes seemedinterminable. No wonder London mayor Boris Johnson looked souncomfortable beforehand. He must have known what was coming.&lt;br /&gt;　　　I have two tips for you, Boris. Keep your hands out of yourpockets when attending high-profile events like this, and secondlyfire whoever responsible for this fiasco and hire proper showbizprofessionals – from Las Vegas if necessary – to handle the openingand closing ceremonies in 2012. Another toe-curling embarrassmentlike this would be unendurable.　　Well done China on a fantastic organisation of the games whichwas flawless and Team GB for their medal haul. Thank you China forputting the U.S into second place.&lt;br /&gt;　　Well done, China! You have given the world a magnificent Olympicsdespite the efforts of the chattering classes in the Western mediato portray you as some Stalinist state.　&lt;br /&gt;　The female who hugged Yao Ming was Lauren Jackson, an Australiabasketball player playing in WNBA. After Yao entered NBA, there wasrumor she had a crush on Yao, but Yao turned her down. There weremany gossip about them.　&lt;br /&gt;　So, seeing them hugging at the closing ceremony really reallymade me laugh, because 4 years ago, at the closing ceremony ofAthens Olympic, Yao and his girlfriend 'Ye Li' came out togetherand that was the first time their relationship went public.&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-1820452690933289533?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/1820452690933289533'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/1820452690933289533'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/london-8-minutes.html' title='London 8 minutes'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-6136492207827679356</id><published>2008-10-18T01:00:00.001-07:00</published><updated>2008-10-18T01:00:31.759-07:00</updated><title type='text'>BUCCAL CELL DNA PREPS</title><content type='html'>Extract the DNA within one week of receiving samples. Samples that have been processed should be frozen to prevent degradation. Freeze samples between use each day.1. Add 600 ml of 50 mM NaOH to a 1.5 ml eppendorf tube and insert brush in tube. (Clip off handle of brush with wire cutters so tube can be closed. Cutters should be rinsed with EtOH between samples to prevent contamination.)2. Vortex thoroughly to mix, for at least 10 s.3. Heat to 95∫C for 5 min.4. Spin briefly to pool condensation.5. Remove brush with tweezers (tweezers should be rinsed with ethanol between samples to prevent contamination) and add 60 ml 1M Tris pH 8.0 to tube. Vortex to mix for at least 10 s.6. Spin for 1 minute at 13,000 rpm and decant supernatant to a clean storage tube.7. Use 2-5 ml of supernatant for standard PCR.&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-6136492207827679356?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/6136492207827679356'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/6136492207827679356'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/buccal-cell-dna-preps.html' title='BUCCAL CELL DNA PREPS'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-2935983023135369386</id><published>2008-10-18T00:59:00.000-07:00</published><updated>2008-10-18T01:00:02.597-07:00</updated><title type='text'>DNA 50th anniversary</title><content type='html'>James Watson and Francis Crick unveiled their model for the structure of DNA in the journal Nature fifty years ago this week. To celebrate, we looks back the key scientific achievements of the twentieth century, forward to DNA's future, and around at the double helix's place in biology.Protocol for extracting DNA from ES Cells, starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA. NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this way!Lysis buffer: ( 100 ml Recipe )10 mM Tris-HCl pH7.5 - 0.5 ml of 2M10 mM EDTA - 2 ml of 0.5M10 mM NaCl - 0.2 ml of 5M0.5% (w/v) Sarkosyl - 0.5gm (N-Lauroylsarcosine, Sigma # L-9150)1mg/ml Proteinase K - add fresh each time (stored in freezer).1. Add 250µl Lysis Buffer (with proteinase K added) to each well.2. Incubate the plate in a sealed humid container at 60°ree;C for 1 hour. (Meanwhile, label eppy tubes!)3. Transfer the contents of each well into a separate 1.5ml eppendorf tube (µ-cent. tube).4. Continue lysing the cells for 2 more hours. (60°ree;C). (Float tubes in waterbath- don't need tupperware).5. Add an equal volume (250 µl) of phenol:chloroform (phenol: chloroform: isoamylalcohol, 25:24:1).(The pH of the phenol:chloroform needs to be ~7.8 - 8.0, or else the DNA will partition into the organic phase).6. Mix the contents of the tube until an emulsion forms. (Shake by hand for ~1 min, (or vortex ~2sec),because vortexing shears long (genomic) DNA).7. Centrifuge 5-10 min in a microfuge at TRm. (The phases should be well-separated).8. Transfer (pipet) the aqueous phase (the top phase) to a fresh tube. (Toss the interface/organic phase-- chemical waste in hood).9. Repeat steps 5-8 until no protein is visible at the interface of the aqueous and organic phases.10. Add an equal volume (250 µl) of chloroform and repeat steps 6-8.11. Optional: Repeat chloroform extraction. (The chloroform extraction removes the traces of phenol).12. Add 1/25th volume of 5M NaCl (10µl of 5M NaCl for 250 µl ), for a final concentration of 0.2M NaCl. Mix well. (use NaCl rather than other salts because of detergent in lysis buffer).13. Add exactly 2 volumes of ice-cold ethanol and again mix the solution well.14. Store the ethanolic solution on ice for 30 min to precipitate the DNA. (can be stored indefinitely at 0°ree;C or at -20°ree;C).15. Centrifuge at 12,000xg for 10 min, 0°ree;C. (Try 4°ree;C, microfuge at max (16,000), 10 min). (Remember to point tube hinges out, to locate pellet even if it's invisible).16. Suck off the supernatant (dispo pipet tip, with vacuum flask). Do not disturb the pellet. Vacuum droplets from walls of tube as well.17. Half fill the tube with 70% EtOH and re-spin for 2 minutes at 4°ree;C.18. Suck off supernatant as in step 16.19. Store the open tube on the bench at TRm until the last traces of fluid have evaporated. (i.e.: Air dry).20. Dissolve the DNA in 30µl (or 50 µl, and use half of sample) of 10mM Tris pH 8.5 (or TE). (pipet around the walls of the tube to dissolve the DNA off the walls of the tube). Let dissolve for at least an hour at 37°ree;C.21. Restriction digest:- DNA in Tris pH8.5, from above protocol- restriction enzyme- restriction enzyme buffer- X (BSA if required by enzyme)- spermidine (to a final conc. of 1mM; stored in freezer)- (also add: ) DTT to a final conc. of 1mM Final volume = 40µl or less. Digest overnight at 37°ree;C.Next: Run gels; Southern blot and hybridize. Shirley Reynolds&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-2935983023135369386?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/2935983023135369386'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/2935983023135369386'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/dna-50th-anniversary.html' title='DNA 50th anniversary'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-5277047712843920150</id><published>2008-10-18T00:58:00.000-07:00</published><updated>2008-10-18T00:59:26.071-07:00</updated><title type='text'>journal Nature fifty years ago this week. To celebrate, we looks back the key scientific achievements of the</title><content type='html'>Standard English The DNA RevolutionFifty Years after Watson and Crick, the New Questions in GeneticsApril 2003 -- Fifty years ago this week, a one-page report published in the British science journal Nature revolutionized science. In it, James Watson and Francis Crick described the three-dimensional structure of DNA.Knowing DNA's structure immediately solved one of biology's greatest mysteries -- how genetic instructions are passed on from one generation to the next. And it opened the door to what has become the hottest area of medical research -- genetics.Human Genome Project director Francis Collins says, even 50 years later, it's impossible to overstate the importance of knowing the structure of DNA:"It is so intertwined in every bit of what we do experimentally, in terms of perceiving our own position in the scheme of life on this planet. It has become one of those givens that is so central to your thinking that you stop thinking about it, but if somebody took it away from you, your whole intellectual foundation would collapse, and it would be unimaginable what we would be doing now if we didn't know about the double helix."To mark the 50th anniversary of Watson and Crick's paper, NPR presents a series of reports on how the discovery has changed research, and what the new biomedical frontiers are.China Marks 34th Earth Day with Focus on Resources Protection Special EnglishOverseas Chinese Return Home For The Good LifeWhen Michael Lee left China in 1986 to pursue an education in the United States, he was swapping a land of poverty for a land of plenty. Today, he is far from sure that's still the case. Along with an increasing number of other well educated Chinese living in America, Lee can't wait to return home as China, with its turbo-charged economic growth, offers better job prospects than the United States. Even the outbreak of the deadly SARS virus in Hong Kong and the mainland does not appear to be a deterrent.Lee said he quit his job to earn an MBA at MIT to help him get on the corporate fast-track back home. He envies his former classmates from a top university in China, as they now hold top corporate positions.To be sure, there are still more Chinese coming into the United States than are returning, but a recent survey found that 80 percent of Chinese students now studying abroad said they would like to go back.Overall, China still suffers more of a brain drain than a brain gain: In the last 25 years, only about a quarter of the students have returned home after graduation, according to the Chinese Ministry of Education.But the number of "sea turtles," the nickname for returnees to China, is growing rapidly. In 2002, 180,000 Chinese students went back, up 47 percent from 2001.Lee and others like him have little trouble finding jobs in China given their U.S.-acquired qualifications, fluency in English and hands-on business experience, say recruiters for U.S. companies.They are much in demand, and the need is immediate, China attracted more foreign direct investment than any other country last year, and an increasing number of U.S. companies, after many years of losing money there, are now turning a profit.Salary levels in China have soared in recent years, with some top lawyers earning as much as 1 million US dollars a year and some engineers in the range of 100 thousand dollars. In a country where the average income is still less than 1/20th of that in the United States, returnees feel that even a reduced salary would allow them a better life style because the cost of living is lower.Tuesday is the 34th World Earth day, which China has given the theme of "treat the earth better and better protect resources."An official with the Ministry of Land and Resources says at a ceremony in Beijing that China is strengthening the protection of resources through the strict implementation of relevant laws.China has drawn up several laws and regulations over the past few years concerning land resource management and coal protection. Meanwhile, investigation work has also been intensified on land resources and new resource explorations.China's per-capita farmland area makes up less than 50 percent of the world's average, and with its huge population and rapid economic development, the country is also suffering a shortage of mining resources&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-5277047712843920150?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/5277047712843920150'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/5277047712843920150'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/journal-nature-fifty-years-ago-this.html' title='journal Nature fifty years ago this week. To celebrate, we looks back the key scientific achievements of the'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-8080781546277971489</id><published>2008-10-18T00:57:00.001-07:00</published><updated>2008-10-18T00:57:52.275-07:00</updated><title type='text'>2008 Lamborghini Reventón</title><content type='html'>&lt;a href="http://l.yimg.com/img.autos.yahoo.com/i/articles/2007_frankfurt_auto_show_articles_2/2008_lamborghini_reventon/lamborghinireventon_l.jpg"&gt;&lt;/a&gt;&lt;br /&gt;Remember the recent Pininfarina Ferrari P4/5 and Maserati GS Zagato? Well, Lamborghini is now building a specialty car of its own: the Reventón, a limited-edition version to be sold to 20 lucky customers as a designer piece.&lt;br /&gt;Deciding not to wait until a coachbuilder picks up a Murciélago to convert it into something different,Lamborghini decided it could do the job better and started work on project code LP640/2, aka the M.O.A., which stands for "Meanest of All." Spokesman Dominik Hoberg said the designation was decided on because the car is expected to have "at least" 650 bhp.&lt;br /&gt;However, we now know the final name of the car to be the 2008 Lamborghini Reventón.We all agree that the look is stunning. All body parts are new, except for the windshield. Edgy, metallic-gray carbon panels form an extreme appearance inspired by modern aircraft design.&lt;br /&gt;The Reventón sports LED lighting front and rear, an all-new fuel inlet and black alloy wheels topped with carbon blades.True to stock dimensions, the Reventón was designed in-house by Centro Stile Lamborghini and "embodies a compression of the brand's DNA," as CEO Stephan Winkelmann points out.&lt;br /&gt;Underneath, the Reventón is all Murciélago with a 6.5-liter V-12 drivetrain.The interior is as special as the exterior. Instead of the stock instrumentation, you'll find functional 3D displays including a g-force meter. By pressing a button, instruments can be changed to a classic analog readout.The 20 slated for production next spring carry a price tag of €1 million. Eleven cars are destined for the U.S.&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-8080781546277971489?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/8080781546277971489'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/8080781546277971489'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/2008-lamborghini-reventn.html' title='2008 Lamborghini Reventón'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-8908825432589279445</id><published>2008-10-18T00:56:00.001-07:00</published><updated>2008-10-18T00:56:43.154-07:00</updated><title type='text'>listening</title><content type='html'>Police in Pompano Beach, Florida have captured the convicted robber who was being taken to court when a Sheriff's deputy was shot and killed. Authorities say Michael Mazza was found with the deputy's gun and there may have been accomplices.2.&lt;br /&gt;President Bush greeted French President Nicolas Sarkozy at the home of George Washington. The two leaders toured the first President's home in Mount Vernon, Virginia and talked of the friendship between the US and France. Earlier Sarkozy addressed a joint session of Congress.3.Televangelist Pat Robertson says Republican Rudy Giuliani will cast a hopeful vision for all Americans. The founder of the Christian Coalition endorsed the presidential candidate. The former New York Mayor's support of abortion and gay rights conflicts with the traditional values of evangelical conservatives.4. Discovery is back on earth.&lt;br /&gt;The space shuttle touched down in Florida after a fifteen-day mission. Discovery landed after safely crossing the continent in the first coast-to-coast reentry since the Columbia disaster.You wanna get on TV? 'In your dreams!' That phrase came in during the 1990s. It meant someone is being unrealistic, very optimistic, very hopeful.Any circumstances in which expectations are raised - in your dreams! It mixes two senses of the word 'dream' - what happens when you're asleep, of course, and the sense of day dream or reverie - it's a very general use.And I've heard it said all over the place in recent months. I've heard it said when in a traffic jam, when the driver thought the road ahead was clearing. 'In your dreams!' said the passenger.&lt;br /&gt;And most interesting of all, I've now heard the phrase being extended with the pronoun changing - you see, 'in your dreams' is the second person, but I've now heard it with a first person and a third person.The other day I heard, 'He's going to try for a part in the movie - in his dreams!' - third person. And then one day somebody said to me, 'I hear you're planning a holiday this year'. And I remember muttering to myself, 'in my dreams'!&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-8908825432589279445?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/8908825432589279445'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/8908825432589279445'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/listening.html' title='listening'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-5002604830022465642</id><published>2008-10-18T00:54:00.000-07:00</published><updated>2008-10-18T00:55:31.424-07:00</updated><title type='text'>Splintering of the Religious Right</title><content type='html'>One sign of division on the right is the civil war breaking out between the business-backed conservatives and religious right leaders. An example was former GOP Majority Leader Dick Armey, saying last week that "James Dobson and his gang of thugs are real nasty bullies," labeling the Focus on the Family leader as engaging in "high demagoguery." Since Armey is now President of FreedomWorks, one of the key corporate-backed lobbying groups in DC and in statehouses around the country, this is a serious declaration of war between previous political allies.The Evangelical Conflict over Climate Change:&lt;br /&gt;But the most serious conflicts are now happening within the religiously conservative movement itself. A clear example was when evangelical leaders this year created the Evangelical Climate Initiative to address climate change and clean up our environment, based around a statement signed by 86 initial evangelical leaders, including Rev. Dr. Leith Anderson, Former President, National Association of Evangelicals (NAE), Rev. Ron Sider, President, Evangelicals for Social Action and Rick Warren, pastor of Saddleback Church and author of mega-seller The Purpose Driven Life.&lt;br /&gt;This was organized by the even older Evangelical Environmental Network, which includes a range of groups including the Texas Baptist Convention.Denouncing this effort in an open letter were other rightwing evangelicals tied to the more traditional political rightwing, including James Dobson, Richard Land of the Southern Baptist Convention, former Watergate criminal, now religious leader Charles Colson, Lou Sheldo of the Traditional Values Coalition, and Donald Wildmon of the American Family Association.Other Divisions Among the Religious Right: This conflict forced the National Association of Evangelicals (NAE), which represents 51 denominations and 10 million people, to abstain from taking a stand on global warming. In a sign of other divisions, the NAE took no position when the war in Iraq started due to the stalemate between anti-war and pro-war factions.Similarly, when Dobson and others on the far right objected to US funding for the Global Fund to Fight AIDS, Tuberculosis and Malaria, because a small portion of its funds go to distributing condoms and providing clean needles to drug addicts, evangelical leaders like Rick Warren and even Pat Robertson stood up to Dobson in defense of the Global Fund as critical for saving lives around the world.The "Other" Baptists: As the Southern Baptist Convention moved sharply to the theological and political right in the last few decades, dissent has broken out throughout its institutions. Baptist colleges have cut ties to the Southern Baptist Convention to protect free thought on their campuses. In 1991, a large number of Southern Baptists formed the Cooperative Baptist Fellowship, including 1800 partner churches and 13 theology schools and seminaries, as an alternative to the rigid fundamentalism of the SBC.&lt;br /&gt;An even more progressive group of Southern Baptists have broken off to form Mainstream Baptists.Most significantly, leaders of these dissident Southern Baptist groups have begun a dialogue with the northern American Baptists (from which the Southern Baptists split in the 19th century over the issue of slavery) and with the African-American Baptist denominations. This year, former President Jimmy Carter (a former Southern Baptist himself) convened a meeting where various Baptist leaders committed to exploring building a North American Baptist Covenant dedicated to "promote peace with justice, to feed the hungry, clothe the naked, shelter the homeless, care for the sick and the marginalized, welcome the strangers among us, and promote religious liberty and respect for religious diversity." Significantly, all of these Baptist groups together include 20 million members, more than the 16 million members remaining in the Southern Baptists.&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-5002604830022465642?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/5002604830022465642'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/5002604830022465642'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/splintering-of-religious-right.html' title='Splintering of the Religious Right'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-2016505840257386894</id><published>2008-10-18T00:53:00.000-07:00</published><updated>2008-10-18T00:54:26.735-07:00</updated><title type='text'>Economic Growth</title><content type='html'>We live in a global economy where corporations have little loyalty to maintaining decent paying jobs in the United States. Failed policies of corporate welfare and tax subsidies to the already wealthy do little but accelerate job flight and growing economic inequality.&lt;br /&gt;Instead of corporate welfare, government should Invest Tax Dollars in Local Assets that generate long-term growth in the economy, includingUniversities and research labs doing technology research;Investments in education, especially for children, where the long-term economic returns multiply over the years;&lt;br /&gt;Transit and physical infrastructure to enhance local commerce;Recreational Facilities, from arts to parks to sports, that should serve the multiple goals of improving the local quality of life, helping attract skilled workers, and enhancing tourism in the community.One key source of investment funds for the private sector should be using Public Pension Funds for Economic Growth. Such funds now control $2.7 trillion in financial assets across the country and investing a significant part of those assets in local economies would bolster the retirement future for our public workforce by assuring a strong economy, local jobs and tax base in the future. Such pension fund investments should include:local venture capital fundssmall business loan fundspartnership with state universities spinning off commercial venturesaffordable housinginvestments in underserved communitiesSince the United States cannot under-price the global competition, we also need regulatory and tax policies that encourage High Road Workplace Practices that preserve high wages through higher productivity.Government contracts and development funds should support Living Wage jobs and long-term reinvestments in the community.&lt;br /&gt;Community Redevelopment programs should encourage maximum use of all our human and physical assets by promoting investments in communities that have lost jobs.Tax Policy should cut the tax burden on productive work, while redirecting taxes to discourage wasteful corporate speculation. Finally, we need to understand that prosperity is more than the limited measures of goods sold embodied in the traditional GDP growth measures.&lt;br /&gt;We need to Redefine Growth Measures to account for the environmental costs and unpaid labor (such as taking care of the home) that is ignored in such measures to define policies that truly maximize family income and well-being. Environmentally Sustainable Growth must be the goal to avoid consumption today coming at the expense of our children's health.&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-2016505840257386894?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/2016505840257386894'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/2016505840257386894'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/economic-growth.html' title='Economic Growth'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-8106637177115214249</id><published>2008-10-18T00:52:00.000-07:00</published><updated>2008-10-18T00:53:06.399-07:00</updated><title type='text'>The passage of time</title><content type='html'>Facing the passage of time, it has brought people should be thick bedding, it is also a very great spring, when we remember yesterday, we put bombs spring to life of another high.Since a person Guaguazhuidi seconds of the second, he would have a "Yesterday." Since a person with ideas, he would be no end to think of yesterday, every person in meditation yesterday when a mature thi&lt;a name="entrymore"&gt;&lt;/a&gt;nking.&lt;br /&gt;Traversed Yesterday, the memories of yesterday, thinking of yesterday……Remember yesterday! We have these 15, six-year-old boy who had a colorful childhood, how happy childhood is a hassle-free day ah. Remember that with partners in the kindergarten play; remember that close to the lovely small rabbit; remember that parents in the Children's Day took me to travel…… countless "that a" cutting into a site of the screen let me unforgettable.Remember yesterday!&lt;br /&gt;We will recall that time and time to commit the folly of their own; crash of the students are quietly with a pen apologize fled; examination and take the very poor parents signed the names to a prank-a-friend crying…… With regret, that a clear pieces of the past surfaced in front of me, with embarrassment, I am in real life who do not ponder the unpleasant past.Yesterday, the matter is over, as Pushkin said, "If the sun and missed flow Xialei, then you have to miss the stars." Indeed, but for fear Who would "miss" and dispel the idea remember yesterday » Yesterday, for a person that is a part of life, so when we are facing the passage of time, we would like to give it endless and recalled thinking. The terrible loss of memory is because people's minds to the past does not exist, and not always thinking recalled yesterday, with the loss of memory of what is the sameFacing the passage of time, it has brought people should be thick bedding, it is also a very great spring, when we remember yesterday, we put bombs spring to life of another high. Remember yesterday!&lt;br /&gt;We have to remember the youth yesterday. Yesterday, the joy and pain, the fortunate and unfortunate yesterday, yesterday's successes and failures. Yesterday, every one of the things we should seriously look at. In reading the passage of time, we have another starting point, so the thinking through yesterday, to mature……&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-8106637177115214249?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/8106637177115214249'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/8106637177115214249'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/passage-of-time.html' title='The passage of time'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-4675026897041803539</id><published>2008-10-18T00:50:00.000-07:00</published><updated>2008-10-18T00:51:53.422-07:00</updated><title type='text'>The Seventh Day &amp; Creation</title><content type='html'>By God here means God in His Triune capacity, FOR, AS HAS BEEN WELL SAID, God the FATHER created the matter, God the SON took the matter and made the worlds and all that exists upon them, and then God the HOLY SPIRIT breathed the “Breath of Life” into the things that have life. John 1:1-3, Eph 3:9, Col. 1:12-16. God rested because His work was finished. That is the only justifiable reason for resting.&lt;br /&gt;God rested because His work was not only finished but was GOOD. There could be no reflection upon it. But when God’s perfect work was marred by sin, by the “Fall of Man,” His “Sabbath Rest” was broken. As soon as man fell it was necessary for God to resume His work, this time not to continue the creation of material things, but for the purpose of the redemption of man that he might become a “NEW CREATION” in Christ Jesus. 2 Cor. 5:17. So Jesus, in explaining His mission said –"My Father is working until now [in creation], and I Myself am working [now in REDEMPTION]." John 5:17.In Gen. 2:1-3, we are simply told that God rested from His work of creating on the ‘SEVENTH Day.” The Day is not called a SABBATH Day.&lt;br /&gt;We are told that God BLESSED and SANCTIFIED it. That is, set it apart as a REST DAY. We are not told the length of the “SEVENTH Day” of the Creative Week, but it should have corresponded with the length of the other days, if sin had not shortened it.THE SABBATHIf God instituted the “Sabbath” before the “Fall of Man,” it seems strange that the fact is not recorded in Genesis, and that Adam was not told to observe it. Nowhere in the Book of Genesis do we read of Adam, or any of his descendants, or Noah, or Abraham observing the Sabbath. The only hint we have of a “seven-fold” division of days is found in Gen. 7:4, 10, when seven days of grace were granted before the Flood came, and in Gen. 8:8-12, where a seven-day period elapsed between the sending forth of the dove.&lt;br /&gt; The first place we read of the Sabbath is in Ex. 16:23-26, in connection with the gathering of the manna -- Six days you shall gather it, but on the seventh day, the Sabbath, there will be none." Here we have the “SEVENTH” day designated as “THE SABBATH.” That the “Seventh Day” of the “Creative Week” was a type of the Sabbath is clear from Ex. 20:11 – “For in six days the LORD made the heavens and the earth, the sea and all that is in them, and rested on the seventh day; therefore the LORD blessed the Sabbath day and made it holy.” But we have no evidence that the Sabbath was commanded to be observed until after the Exodus, and the reason is clear. God’s “Rest Day” was broken by the “Fall of Man,” and there could be no “rest” until redemption was brought in, and this was typically brought in by the redemption of the Children of Israel from Egypt through the offering of the “Passover Lamb,” a type of Christ. The purpose of their deliverance was that they might find rest in Canaan from the weary toil and slavery of Egypt. Deu. 5:15.When a few weeks later the “Ten Commandments” were given on Mt. Sinai the Lord said to Israel, “Remember the Sabbath day, to keep it holy,” (Ex. 20:8), the Sabbath Day they were to remember was not the “Seventh Day” on which God rested, but the “Day” that God had appointed as the “Sabbath Day” at the time of the giving of the manna.The command to observe the Sabbath was given to Israel EXCLUSIVELY. It was not given to the Gentiles. It was given to Israel as the “SIGN” of the “Mosaic Covenant.” “But as for you, speak to the sons of Israel, saying, 'You shall surely observe My Sabbaths; for this is a sign between Me and you throughout your generations.” Ex. 31:13; Ezek. 20:12, 19-21.&lt;br /&gt;The Sabbath Day then belongs to the Jews alone and is not binding on the Gentiles (the world), or on the Church (Christians). Nowhere in the Bible do you find God finding fault with any nation or people, except the Jewish nation, for not observing the Sabbath. As a Jewish ordinance it has never been abrogated, changed, or transferred to any other day of the week, or to any other people. It is now in abeyance as foretold in Hosea 2:11 it would be. It is to be resumed when the Jews are nationally restored to their own land. Isa. 66:23. Ezek. 44:24; 46:1-3.If this be true, then the “Sabbath’ does not belong to the Church, and is not to be observed by Christians, for the “Sabbath Day” is a part of “THE LAW,” and Christians are nut under “LAW,” but under “GRACE.” Rom 6:14. In his letter to the Galatian Christians Paul reproved them for going back to the “Law,” and declared that those who did so were “under the CURSE.” Gal 3:10. “But now that you have come to know God, or rather to be known by God, how is it that you turn back again to the weak and worthless elemental things, to which you desire to be enslaved all over again? You observe days [Sabbath and Feast Days] and months and seasons and years. I fear for you, that perhaps I have labored over you in vain.” Gal. 4:9-11. “Therefore no one is to act as your judge in regard to food or drink or in respect to a festival or a new moon or a Sabbath day—Col. 2:16. If Christians are under obligation to keep the “Jewish Sabbath,” then they are under the Jewish “Ceremonial Law” and should observe all the ordinances and Feast Days of the Jewish Ritual.As an institution of Judaism, the Sabbath, with all the “Feast Days” and other ritualistic ceremonies and offerings of Judaism, ceased to function with the close of the Jewish Dispensation. The JEWISH Sabbath was not changed to the CHRISTIAN Sabbath, any more than “Circumcision” was changed to “Baptism.” There is no such thing as the “CHRISTIAN Sabbath.” “Sabbath” has to do with LAW, and “Christian” with GRACE, and to join “LAW” and “GRACE” is to unite what God has forever separated.After the Resurrection, Christ and His Disciples never met on the “Sabbath” but on the “FIRST DAY of the week.” John 20:1, 19. Acts 20:7. 1 Cor. 16:2. It is true that they went into the Jewish Synagogues on the Sabbath, but not to worship, but that they might have opportunity to preach the Gospel. The “First Day of the Week” is the day to be observed for rest and worship by the Christian Church. It is prefigured in the Old Testament as the “EIGHTH DAY,” or the “DAY AFTER THE SABBATH.” “You shall bring in the sheaf of the first fruits of your harvest to the priest. 'He shall wave the sheaf before the LORD for you to be accepted; on the day after the Sabbath the priest shall wave it.’” Lev. 23:10-11.&lt;br /&gt; What did that “First Fruits” typify? Read 1 Cor. 15:20 – “But now Christ has been raised from the dead, the first fruits of those who are asleep.” When did Christ rise from the dead and become the “FIRST FRUITS?” Not on the “Sabbath,” for He lay dead in the tomb on that day, but on the “FIRST DAY OF THE WEEK,” on the “DAY AFTER THE SABBATH.”The fact that the “Birthday” of the Church was at Pentecost, and that fell on the “First Day of the Week,” is further proof that the Church should keep the “First Day of the Week” and not the “Seventh” day or “SABBATH.”The Jewish Sabbath links man with the “Old Dispensation,” the “First Day of the Week” links man with the “New.” It is claimed that the Roman Catholic Church changed the day of rest from the “Seventh Day” to the “First Day of the Week,” but the claim is false, for the Papacy did not exist until a long time after the “First Day of the Week” had become a fixed day for Christian worship. It is a noteworthy fact that the whole of the “Ten Commandments” (Ex. 20:1-17) are reaffirmed in the New Testament, except the “Fourth Commandment” regarding the Sabbath. Rom. 13:8-10, Eph. 6:1-2, James 5:12, 1 John 5:21. Why this omission if the Law of the “Sabbath” is still in force?&lt;br /&gt; It is called the “LORD’S DAY.” It belongs to Him. It is not called a “rest day” in the Bible. It is a day that should be filled with worship and service and holy activity. It is not a day to be spent in laziness or pleasure, or the giving of sacred concerts and the discussion of worldly betterment schemes, but a day for the teaching and preaching of the Word of God.&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-4675026897041803539?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/4675026897041803539'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/4675026897041803539'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/seventh-day-creation.html' title='The Seventh Day &amp; Creation'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-364131870939177695</id><published>2008-10-18T00:49:00.001-07:00</published><updated>2008-10-18T00:49:59.455-07:00</updated><title type='text'>Antibody Conjugation Service</title><content type='html'>Antibody Conjugation Service$799/1mg, the most competitive price for antibody conjugation!BioChain provides the premium quality Antibody Conjugation Service with a 1-2 week turn around time.&lt;br /&gt;The Service is focus on HRP and Biotin.BiotinBiotin is the conjugate of choice if the antibody is going to be used in a variety of applications. Biotin is a small molecule that has little effect on an antibody's immunoreactivity. Its small size makes biotin an excellent tag for your antibody in most techniques. Biotin binds tightly to avidin or streptavidin, which are available enzyme conjugated, making your biotinylated antibody useful in most immunological procedures.The disadvantage of a biotinylated antibody over an enzyme or fluorescent conjugated antibody is that there is an additional step involved in your assay.&lt;br /&gt;HRPEnzyme conjugates give a highly selective and sensitive immuno-reagent. By directly labelling your primary antibody you eliminate steps and washes. Horseradish Peroxidase (HRP) is a stable enzyme suitable for ELISA, Western Blot and immunocytochemistry.&lt;br /&gt;HRP has low endogenous activity and a high turnover rate, producing its coloured end product in a short time period.Customers need to supply a minimum quantity of 1 mg of antibody is required for all conjugationsThis FDA standard Frozen Tissue Array is designed in close conformance with FDA guideline for meeting requirements for IHC (Immunohistochemistry) and IVD (In vitro Diagnostic Devices) certification and also European CE Mark. This tissue array has two slides which contain 30 different normal tissue types and 3 donors per tissue type.&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-364131870939177695?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/364131870939177695'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/364131870939177695'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/antibody-conjugation-service.html' title='Antibody Conjugation Service'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-7116610919170865494</id><published>2008-10-18T00:47:00.000-07:00</published><updated>2008-10-18T00:49:09.402-07:00</updated><title type='text'>Custom Recombinant Protein</title><content type='html'>BioChain's Recombinant Protein Expression Service is a complete solution for your needs, helping your organization to deal with the protein bottleneck. Protein Expression Service combines a variety of expression systems (bacterial and baculorvirus), across a range of scales, with a full range of purification options, providing you with the material that you require for your application. The service spans vector construction through to purification of ready to use protein and can be tailored to meet your exact requirements.&lt;br /&gt;Construct GenerationClone coding sequence into one of a wide range of expression vectorsThe expression construct will be fully sequence verifiedPreparation of plasmid construct DNA Assessment and OptimizationIntroduce plasmid construct DNA into bacterial/baculovirusSelection of over-expressing cellsSmall scale expression and purification study assessed by SDS-PAGE and Western blot (anti-tag or specific antiserum) analysisEstimation of protein yield Scale-up and PurificationLarge-scale growth of over-expressing cells&lt;br /&gt;Cells are harvested and lysedProtein typically purified from cleared lysate via purification tagFurther purification to remove any contaminants e.g. gel filtrationSDS-PAGE and Western blot analysis of purified proteinDetermination of protein concentrationDelivery of purified protein ready for use in your application Recombinant Protein Production TeamYour project will be managed by a team with extensive experience in the expression and purification of a wide range of proteins in both bacterial and mammalian systems.&lt;br /&gt;All of our services are fully supported by scientists who directly manage your project and are on hand to develop and support a service that meets your exact requirements.&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-7116610919170865494?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/7116610919170865494'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/7116610919170865494'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/custom-recombinant-protein_18.html' title='Custom Recombinant Protein'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry><entry><id>tag:blogger.com,1999:blog-3911178684277112424.post-9159856087902847822</id><published>2008-10-18T00:45:00.000-07:00</published><updated>2008-10-18T00:46:52.131-07:00</updated><title type='text'>Custom Recombinant Protein</title><content type='html'>BioChain's Recombinant Protein Expression Service is a complete solution for your needs, helping your organization to deal with the protein bottleneck. Protein Expression Service combines a variety of expression systems (bacterial and baculorvirus), across a range of scales, with a full range of purification options, providing you with the material that you require for your application. The service spans vector construction through to purification of ready to use protein and can be tailored to meet your exact requirements.&lt;br /&gt;Construct GenerationClone coding sequence into one of a wide range of expression vectorsThe expression construct will be fully sequence verifiedPreparation of plasmid construct DNA Assessment and OptimizationIntroduce plasmid construct DNA into bacterial/baculovirusSelection of over-expressing cellsSmall scale expression and purification study assessed by SDS-PAGE and Western blot (anti-tag or specific antiserum) analysisEstimation of protein yield Scale-up and PurificationLarge-scale growth of over-expressing cellsCells are harvested and lysedProtein typically purified from cleared lysate via purification tag&lt;br /&gt;Further purification to remove any contaminants e.g. gel filtrationSDS-PAGE and Western blot analysis of purified proteinDetermination of protein concentrationDelivery of purified protein ready for use in your application Recombinant Protein Production TeamYour project will be managed by a team with extensive experience in the expression and purification of a wide range of proteins in both bacterial and mammalian systems.&lt;br /&gt;All of our services are fully supported by scientists who directly manage your project and are on hand to develop and support a service that meets your exact requirements.&lt;div class="blogger-post-footer"&gt;&lt;img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/3911178684277112424-9159856087902847822?l=tj080816.blogspot.com' alt='' /&gt;&lt;/div&gt;</content><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/9159856087902847822'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/3911178684277112424/posts/default/9159856087902847822'/><link rel='alternate' type='text/html' href='http://tj080816.blogspot.com/2008/10/custom-recombinant-protein.html' title='Custom Recombinant Protein'/><author><name>sexhome</name><uri>http://www.blogger.com/profile/09775024730302748669</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author></entry></feed>
