BioChain's Recombinant Protein Expression Service is a complete solution for your needs, helping your organization to deal with the protein bottleneck. Protein Expression Service combines a variety of expression systems (bacterial and baculorvirus), across a range of scales, with a full range of purification options, providing you with the material that you require for your application. The service spans vector construction through to purification of ready to use protein and can be tailored to meet your exact requirements.
Construct GenerationClone coding sequence into one of a wide range of expression vectorsThe expression construct will be fully sequence verifiedPreparation of plasmid construct DNA Assessment and OptimizationIntroduce plasmid construct DNA into bacterial/baculovirusSelection of over-expressing cellsSmall scale expression and purification study assessed by SDS-PAGE and Western blot (anti-tag or specific antiserum) analysisEstimation of protein yield Scale-up and PurificationLarge-scale growth of over-expressing cellsCells are harvested and lysedProtein typically purified from cleared lysate via purification tag
Further purification to remove any contaminants e.g. gel filtrationSDS-PAGE and Western blot analysis of purified proteinDetermination of protein concentrationDelivery of purified protein ready for use in your application Recombinant Protein Production TeamYour project will be managed by a team with extensive experience in the expression and purification of a wide range of proteins in both bacterial and mammalian systems.
All of our services are fully supported by scientists who directly manage your project and are on hand to develop and support a service that meets your exact requirements.
